Endogenous CCL2 (monocyte chemotactic protein-1) modulates human immunodeficiency virus type-1 replication and affects cytoskeleton organization in human monocyte-derived macrophages

Laura Fantuzzi, Francesca Spadaro, Giuliana Vallanti, Irene Canini, Carlo Ramoni, Elisa Vicenzi, Filippo Belardelli, Guido Poli, Sandra Gessani

Research output: Contribution to journalArticle

Abstract

CC chemokine ligand 2 (CCL2) is constitutively expressed at high levels in human peripheral blood monocytes, and its expression is further up-modulated during their differentiation into macrophages as well as in the course of HIV infection. To investigate the role of endogenous CCL2 on HIV replication and macrophage function, CCL2's activity was neutralized by specific antibodies. Infection of monocyte-derived macrophages with laboratory-adapted HIV-1 or primary viral isolates in the continuous presence of anti-CCL2 antibody resulted in significantly lower p24 Gag antigen release with respect to control cultures. Interestingly, CCL2 neutralization did not affect the early steps of the HIV life cycle but resulted in the intracellular accumulation of p24 Gag antigen. Simultaneously, remarkable changes in cell morphology and size occurred in cell cultures maintained in the presence of anti-CCL2 antibody. These results suggest that CCL2 may represent an autocrine factor important for enhancing virion production likely by affecting the macrophage cytoskeleton.

Original languageEnglish
Pages (from-to)2334-2337
Number of pages4
JournalBlood
Volume102
Issue number7
DOIs
Publication statusPublished - Oct 1 2003

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Endogenous CCL2 (monocyte chemotactic protein-1) modulates human immunodeficiency virus type-1 replication and affects cytoskeleton organization in human monocyte-derived macrophages'. Together they form a unique fingerprint.

  • Cite this