Endogenous dopamine release from tuberoinfundibular neurons: Does calmodulin play any role?

G. F. Di Renzo, S. Amoroso, M. Taglialatela, L. Annunziato

Research output: Contribution to journalArticle

Abstract

The possible involvement of calmodulin in the process of endogenous dopamine (DA) release from arcuateperiventricular nuclei-median eminence fragments, containing tuberoinfundibular dopaminergic (TIDA) neurons, has been investigated in an in vitro incubation system. For this purpose the basal and K+-stimulated DA release was examined in the presence and in the absence of the different putative calmodulin antagonists, pimozide, trifluoperazine, penfluridol and N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide (W-7). Trifluoperazine and pimozide in concentrations up to 100 μM were both uneffective in blocking K+-evoked DA release. Penfluridol in doses of 5 and 10 μM, did not prevent 35 mM K+-induced endogenous DA release. It was able to reduce K+-stimulated DA release only at the very large concentration of 100 μM. W-7 added in vitro to the hypothalamic fragments, prevented endogenous DA release evoked by 35 mM K+ in a dose-dependent manner. W-5, a chlorine deficient analogue of W-7, that interacts only weakly with calmodulin, failed to modify K+-stimulated endogenous DA release in doses up to 200 μM. All the putative calmodulin antagonists used in the present study did not induce any change of basal DA release. IN conclusion the fact that most of the agents, except W-7, known to antagonize calmodulin-dependent processes in many biological systems failed to interfere with the release of endogenous DA from TIDA neurons seems to suggest that calmodulin does not play a crucial role in the process of DA release and that the inhibitory effect of W-7 on endogenous DA release may be better attributed to other mechanisms different from its anticalmodulin action.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume333
Issue number3
DOIs
Publication statusPublished - Jul 1986

Keywords

  • Calmodulin
  • Calmodulin antagonists
  • Dopamine
  • Tuberoinfundibular neurons
  • W-7

ASJC Scopus subject areas

  • Pharmacology

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