TY - JOUR
T1 - Endogenous insulin-like growth factors regulate the proliferation of TSH-independent mutants derived from FRTL5 cells
AU - Chieffi, Paolo
AU - Picascia, Antonella
AU - Stanzione, Rosita
AU - Villone, Giovanni
AU - Tramontano, Donatella
PY - 1999/4
Y1 - 1999/4
N2 - TSH-independent mutant clones (M cells) derived from FRTL5 cells, proliferate vigorously in the absence of TSH. The growth of M cells is stimulated by IGF-I in a dose-dependent fashion, but it is not influenced by TSH. Sm1.2, an antibody against IGF-I cross-reacting with IGF-II, significantly decreases basal DNA synthesis in the M cells. Binding of
125I-IGF-I to M cells is significantly lower than that to FRTL5 cells. M cells produce in their culture medium IGF-like peptides which appear to influence their basal DNA synthesis and the availability of type I receptors to bind exogenous IGF-I.
AB - TSH-independent mutant clones (M cells) derived from FRTL5 cells, proliferate vigorously in the absence of TSH. The growth of M cells is stimulated by IGF-I in a dose-dependent fashion, but it is not influenced by TSH. Sm1.2, an antibody against IGF-I cross-reacting with IGF-II, significantly decreases basal DNA synthesis in the M cells. Binding of
125I-IGF-I to M cells is significantly lower than that to FRTL5 cells. M cells produce in their culture medium IGF-like peptides which appear to influence their basal DNA synthesis and the availability of type I receptors to bind exogenous IGF-I.
KW - Cell growth
KW - FRTL5 cells
KW - Somatomedin
KW - Thyroid
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UR - http://www.scopus.com/inward/citedby.url?scp=0032735843&partnerID=8YFLogxK
U2 - 10.1016/S0300-9084(99)80083-2
DO - 10.1016/S0300-9084(99)80083-2
M3 - Article
C2 - 10401671
AN - SCOPUS:0032735843
VL - 81
SP - 367
EP - 371
JO - Biochimie
JF - Biochimie
SN - 0300-9084
IS - 4
ER -