Endogenous insulin-like growth factors regulate the proliferation of TSH-independent mutants derived from FRTL5 cells

Paolo Chieffi, Antonella Picascia, Rosita Stanzione, Giovanni Villone, Donatella Tramontano

Research output: Contribution to journalArticlepeer-review

Abstract

TSH-independent mutant clones (M cells) derived from FRTL5 cells, proliferate vigorously in the absence of TSH. The growth of M cells is stimulated by IGF-I in a dose-dependent fashion, but it is not influenced by TSH. Sm1.2, an antibody against IGF-I cross-reacting with IGF-II, significantly decreases basal DNA synthesis in the M cells. Binding of 125I-IGF-I to M cells is significantly lower than that to FRTL5 cells. M cells produce in their culture medium IGF-like peptides which appear to influence their basal DNA synthesis and the availability of type I receptors to bind exogenous IGF-I.

Original languageEnglish
Pages (from-to)367-371
Number of pages5
JournalBiochimie
Volume81
Issue number4
DOIs
Publication statusPublished - Apr 1999

Keywords

  • Cell growth
  • FRTL5 cells
  • Somatomedin
  • Thyroid

ASJC Scopus subject areas

  • Biochemistry

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