Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state

Brian D. Brown, Bernhard Gentner, Alessio Cantore, Silvia Colleoni, Mario Amendola, Anna Zingale, Alessia Baccarini, Giovanna Lazzari, Cesare Galli, Luigi Naldini

Research output: Contribution to journalArticlepeer-review

Abstract

We have shown previously that transgene expression can be suppressed in hematopoietic cells using vectors that are responsive to microRNA (miRNA) regulation. Here we investigate the potential of this approach for more sophisticated control of transgene expression. Analysis of the relationship between miRNA expression levels and target mRNA suppression suggested that suppression depends on a threshold miRNA concentration. Using this information, we generated vectors that rapidly adjust transgene expression in response to changes in miRNA expression. These vectors sharply segregated transgene expression between closely related states of therapeutically relevant cells, including dendritic cells, hematopoietic and embryonic stem cells, and their progeny, allowing positive/negative selection according to the cells' differentiation state. Moreover, two miRNA target sites were combined to restrict transgene expression to a specific cell type in the liver. Notably, the vectors did not detectably perturb endogenous miRNA expression or regulation of natural targets. The properties of miRNA-regulated vectors should allow for safer and more effective therapeutic applications.

Original languageEnglish
Pages (from-to)1457-1467
Number of pages11
JournalNature Biotechnology
Volume25
Issue number12
DOIs
Publication statusPublished - Dec 2007

ASJC Scopus subject areas

  • Microbiology

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