Endogenous nitric oxide production by human monocytic cells regulates LPS-induced TNF production

M. Zinetti, G. Fantuzzi, R. Delgado, E. Di Santo, P. Ghezzi, M. Fratelli

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The ability to produce nitric oxide (NO) of human monocytes macrophages is object of debate. While studying the regulation of tumor necrosis factor (TNF) synthesis induced by endotoxin (LPS) in a human cell line of monocyte origin (THP-1) and in human peripheral blood mononuclear cells (PBMC) we found an indirect evidence of such production. We showed that L-N-monomethyl-arginine (L-NMMA), an inhibitor of NO synthase, and hemoglobin, a chelator of NO, are able to significantly reduce TNF synthesis, indicating that NO production is induced by LPS and contributes to the induction of TNF. Since NO is a known cytostatic agent, we also studied the cytostatic effect of LPS, and demonstrated that it is reverted by L-NMMA. Although we were unable to show any nitrites/nitrates accumulation in the culture media, taken together our data give an indirect evidence of a physiologically relevant LPS-induced NO production in human monocytes-macrophages.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalEuropean Cytokine Network
Volume6
Issue number1
Publication statusPublished - 1995

Fingerprint

Nitric Oxide
Tumor Necrosis Factor-alpha
Monocytes
Macrophages
Cytostatic Agents
Arginine
Chelating Agents
Nitrites
Endotoxins
Nitric Oxide Synthase
Nitrates
Culture Media
Blood Cells
Blood
Cells
Cell Line

Keywords

  • Human monocytes/macrophages
  • Nitric oxide
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Endogenous nitric oxide production by human monocytic cells regulates LPS-induced TNF production. / Zinetti, M.; Fantuzzi, G.; Delgado, R.; Di Santo, E.; Ghezzi, P.; Fratelli, M.

In: European Cytokine Network, Vol. 6, No. 1, 1995, p. 45-48.

Research output: Contribution to journalArticle

Zinetti, M, Fantuzzi, G, Delgado, R, Di Santo, E, Ghezzi, P & Fratelli, M 1995, 'Endogenous nitric oxide production by human monocytic cells regulates LPS-induced TNF production', European Cytokine Network, vol. 6, no. 1, pp. 45-48.
Zinetti, M. ; Fantuzzi, G. ; Delgado, R. ; Di Santo, E. ; Ghezzi, P. ; Fratelli, M. / Endogenous nitric oxide production by human monocytic cells regulates LPS-induced TNF production. In: European Cytokine Network. 1995 ; Vol. 6, No. 1. pp. 45-48.
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