Abstract
Survivin is a bi-functional member of inhibitor of apoptosis protein family, as it is able to both inhibit apoptosis and to regulate cell cycle. We investigated the role of survivin in human keratinocytes under normal conditions and during UVB irradiation. Survivin siRNA decreases proliferation and induces apoptosis in human keratinocytes, in a mode consistent with the mitotic catastrophe. Low doses UVB increase survivin expression at earlier times, while high doses down-regulate survivin level. Low doses UVB induce cell cycle arrest in G2/M, while high doses UVB cause apoptosis. Moreover, overexpression of survivin protects keratinocytes from UVB-induced apoptosis, and silencing of survivin renders keratinocytes more susceptible to UVB-induced cell death. Finally, survivin siRNA increases UVB-induced reduction of cell proliferation. Taken together, these results indicate that survivin plays a critical role in epidermal homeostasis in normal conditions and during UVB exposure, with possible implication in skin carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 464-471 |
| Number of pages | 8 |
| Journal | Experimental Dermatology |
| Volume | 18 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- Apoptosis
- G2/M arrest
- Keratinocytes
- Survivin
- UVB
ASJC Scopus subject areas
- Dermatology
- Molecular Biology
- Biochemistry
Cite this
Endogenous survivin modulates survival and proliferation in UVB-treated human keratinocytes. / Dallaglio, Katiuscia; Palazzo, Elisabetta; Marconi, Alessandra; Dumas, Marc; Truzzi, Francesca; Lotti, Roberta; Bontè, Frederic; Pincelli, Carlo.
In: Experimental Dermatology, Vol. 18, No. 5, 2009, p. 464-471.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endogenous survivin modulates survival and proliferation in UVB-treated human keratinocytes
AU - Dallaglio, Katiuscia
AU - Palazzo, Elisabetta
AU - Marconi, Alessandra
AU - Dumas, Marc
AU - Truzzi, Francesca
AU - Lotti, Roberta
AU - Bontè, Frederic
AU - Pincelli, Carlo
PY - 2009
Y1 - 2009
N2 - Survivin is a bi-functional member of inhibitor of apoptosis protein family, as it is able to both inhibit apoptosis and to regulate cell cycle. We investigated the role of survivin in human keratinocytes under normal conditions and during UVB irradiation. Survivin siRNA decreases proliferation and induces apoptosis in human keratinocytes, in a mode consistent with the mitotic catastrophe. Low doses UVB increase survivin expression at earlier times, while high doses down-regulate survivin level. Low doses UVB induce cell cycle arrest in G2/M, while high doses UVB cause apoptosis. Moreover, overexpression of survivin protects keratinocytes from UVB-induced apoptosis, and silencing of survivin renders keratinocytes more susceptible to UVB-induced cell death. Finally, survivin siRNA increases UVB-induced reduction of cell proliferation. Taken together, these results indicate that survivin plays a critical role in epidermal homeostasis in normal conditions and during UVB exposure, with possible implication in skin carcinogenesis.
AB - Survivin is a bi-functional member of inhibitor of apoptosis protein family, as it is able to both inhibit apoptosis and to regulate cell cycle. We investigated the role of survivin in human keratinocytes under normal conditions and during UVB irradiation. Survivin siRNA decreases proliferation and induces apoptosis in human keratinocytes, in a mode consistent with the mitotic catastrophe. Low doses UVB increase survivin expression at earlier times, while high doses down-regulate survivin level. Low doses UVB induce cell cycle arrest in G2/M, while high doses UVB cause apoptosis. Moreover, overexpression of survivin protects keratinocytes from UVB-induced apoptosis, and silencing of survivin renders keratinocytes more susceptible to UVB-induced cell death. Finally, survivin siRNA increases UVB-induced reduction of cell proliferation. Taken together, these results indicate that survivin plays a critical role in epidermal homeostasis in normal conditions and during UVB exposure, with possible implication in skin carcinogenesis.
KW - Apoptosis
KW - G2/M arrest
KW - Keratinocytes
KW - Survivin
KW - UVB
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UR - http://www.scopus.com/inward/citedby.url?scp=65249097118&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0625.2008.00819.x
DO - 10.1111/j.1600-0625.2008.00819.x
M3 - Article
C2 - 19320741
AN - SCOPUS:65249097118
VL - 18
SP - 464
EP - 471
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
IS - 5
ER -