Objective: To determine whether in vitro induction of endomysial antibodies is an in vitro marker of coeliac toxicity. Design: To determine whether in vitro endomysial antibodies induced by gliadin incubation correlate with histological damage induced by in vitro gliadin challenge. Methods: Small-bowel organ cultures from seven patients with treated coeliac disease were incubated in an organ culture system with gliadin; histological damage was morphometrically evaluated and endomysial antibodies were measured in the organ culture supernatant by indirect immunofluorescence. Results: Although incubation with gliadin caused histological damage, there was no detectable production of endomysial antibody. Conclusions: In vitro, endomysial antibody induction cannot be used as a marker of coeliac toxicity. Endomysial antibodies are not necessary for generating the histological lesion of coeliac disease. (C) 2000 Lippincott Williams and Wilkins.
|Number of pages||4|
|Journal||European Journal of Gastroenterology and Hepatology|
|Publication status||Published - 2000|
- Coeliac disease
- Endomysial antibodies
- Tissue transglutaminase
ASJC Scopus subject areas