Endomysial antibody production is not related to histological damage after in vitro gluten challenge

Federico Biagi, Nick D J Parnell, H. Julia Ellis, Paul J. Ciclitira

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine whether in vitro induction of endomysial antibodies is an in vitro marker of coeliac toxicity. Design: To determine whether in vitro endomysial antibodies induced by gliadin incubation correlate with histological damage induced by in vitro gliadin challenge. Methods: Small-bowel organ cultures from seven patients with treated coeliac disease were incubated in an organ culture system with gliadin; histological damage was morphometrically evaluated and endomysial antibodies were measured in the organ culture supernatant by indirect immunofluorescence. Results: Although incubation with gliadin caused histological damage, there was no detectable production of endomysial antibody. Conclusions: In vitro, endomysial antibody induction cannot be used as a marker of coeliac toxicity. Endomysial antibodies are not necessary for generating the histological lesion of coeliac disease. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)57-60
Number of pages4
JournalEuropean Journal of Gastroenterology and Hepatology
Volume12
Issue number1
Publication statusPublished - 2000

Keywords

  • Coeliac disease
  • Endomysial antibodies
  • Gliadin
  • Tissue transglutaminase

ASJC Scopus subject areas

  • Gastroenterology

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