Endoscopic ultrasound-guided application of a new internally gas-cooled radiofrequency ablation probe in the liver and spleen of an animal model: A preliminary study

S. Carrara, Paolo Giorgio Arcidiacono, L. Albarello, A. Addis, M. D. Enderle, C. Boemo, A. Neugebauer, M. Campagnol, C. Doglioni, P. A. Testoni

Research output: Contribution to journalArticle

Abstract

Background and study aims: In a previous study, a new flexible bipolar hybrid cryotherm probe was applied with success to the pancreas of a living pig. Here we evaluated feasibility, efficacy, and safety of its application to the porcine liver and spleen. Material and methods: Ten applications to the liver and nine to the spleen were performed in pigs. Power input (16-18W) and simultaneous cooling with CO2 (standardized pressure: 675psi) as the cryogenic agent were investigated. Application time varied from 120 seconds to 900 seconds. The ablation area was measured by endoscopic ultrasound (EUS) after ablation (TO), and before euthanasia (T1). Gross pathology (T2) and histology after necropsy represented the gold standard. The interval from treatment to euthanasia was 1 or 2 weeks. Results: For both organs the correlation between EUS and gross pathology was good (correlation coefficient Rliver=0.71; Rspleen=0.73). EUS tended to overestimate the area of the ablated zone. EUS observed a time-dependent ablation area: we demonstrated a positive trend of lesion size (T1) over time in liver tissue (R=0.51 (P=0.1)). In the spleen we found a clear correlation of lesion area T2 and application time (R=0.75, P=0.01). There were no complications. Conclusions: Selective EUS-guided transgastric cryotherm ablation of the liver and spleen in a pig model is feasible and safe. The new bipolar probe creates a time-dependent ablation area without any complications, and opens a field of new potential indications of RF-ablative therapies.

Original languageEnglish
Pages (from-to)759-763
Number of pages5
JournalEndoscopy
Volume40
Issue number9
DOIs
Publication statusPublished - Sep 2008

ASJC Scopus subject areas

  • Medicine (miscellaneous)

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