Endothelial cell injury induced by preservation solutions: A confocal microscopy study

Francesco Alamanni, Alessandro Parolari, Rossana Visigalli, Ovidio Bussolati, Patrizia Rubini, Roberto Sala, Luigi Bonati, Gian Carlo Gazzola, Paolo Biglioli, Valeria Dall'Asta

Research output: Contribution to journalArticlepeer-review


Background. We evaluated the effects of standard preservation solutions on cultured human greater saphenous vein endothelial cells. Methods. Endothelial cells (eight strains) were preincubated for 6 or 24 hours at 4°C in Celsior, Euro-Collins, St. Thomas Hospital II, and University of Wisconsin solutions, reincubated in warm oxygenated culture medium 199, and observed up to 48 hours. Culture viability was assessed through cell counting and confocal microscopy of calcein loaded cells. Results. Incubation in both Euro-Collins and St. Thomas, but not in Celsior or University of Wisconsin solutions, caused significant cells losses and diffuse morphological damages characterized by solution-specific distinctive alterations. Injury caused by 6-hour, but not by 24-hour treatment, was reversible. Conclusions. The incubation with Celsior and University of Wisconsin solutions substantially preserved endothelial viability and proliferative capability. Conversely, a prolonged incubation in either Euro-Collins or St. Thomas solutions caused severe and potentially irreversible damage referable to the induction of, respectively, apoptotic or necrotic changes.

Original languageEnglish
Pages (from-to)1606-1614
Number of pages9
JournalAnnals of Thoracic Surgery
Issue number5
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery


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