Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis

Amelia Casamassimi, Filomena De Nigris, Concetta Schiano, Claudio Napoli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter we will describe the use of the tubular network assay as a useful tool in the evaluation of cancer neoangiogenesis (as reported by Arnaoutova et al. [Angiogenesis 12:267–274, 2009]). Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. A detailed working protocol applicable to the study of any type of endothelial cells is provided below and in addition, details on data interpretation analysis and troubleshooting.

Original languageEnglish
Title of host publicationHandbook of Vascular Biology Techniques
PublisherSpringer Netherlands
Pages13-22
Number of pages10
ISBN (Print)9789401797160, 9789401797153
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Cancer neoangiogenesis
  • Data analysis
  • Human aortic endothelial cells
  • Tube formation

ASJC Scopus subject areas

  • Medicine(all)
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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