Abstract
Problem: To investigate the role of the plasma cofactor for antiphospholipid antibodies in antibody binding to endothelial cells. Method of Study: a) Evaluation of endothelial cell binding of polyclonal and monoclonal anti-β2 glycoprotein I antibodies; and b) study of the effects of antibody binding: adhesion molecule expression. leukocyte adhesion, and interleukin-1β secretion. Results: Anti-β2 glycoprotein I antibodies bind endothelial cell monolayers in vitro by reacting with the cofactor adhered to the cell membranes. Antibody binding induces an upregulation of adhesion molecules, favours leukocyte adherence, and increases interleukin-1β secretion. Interleukin-1β plays an active role to mediate adhesion molecule expression through an autocrine loop, as shown by the inhibitory effect of interleukin 1 receptor antagonist. Conclusions: Antiphospholipid antibodies do react with endothelium through the cofactor adhered to their cell membranes and induces a pro-adhesive cell phenotype.
| Original language | English |
|---|---|
| Pages (from-to) | 212-217 |
| Number of pages | 6 |
| Journal | American Journal of Reproductive Immunology |
| Volume | 38 |
| Issue number | 3 |
| Publication status | Published - 1997 |
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Keywords
- β2 glycoprotein I
- Adhesion molecules
- Antiphospholipid antibodies
- Cytokines
- Endothelial cells
- Thrombosis
ASJC Scopus subject areas
- Immunology
- Obstetrics and Gynaecology
Cite this
Endothelial cells as a target for antiphospholipid antibodies : Role of anti-beta 2 glycoprotein I antibodies. / Del Papa, N.; Raschi, E.; Catelli, L.; Khamashta, M. A.; Ichikawa, K.; Tincani, A.; Balestrieri, G.; Meroni, P. L.
In: American Journal of Reproductive Immunology, Vol. 38, No. 3, 1997, p. 212-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endothelial cells as a target for antiphospholipid antibodies
T2 - Role of anti-beta 2 glycoprotein I antibodies
AU - Del Papa, N.
AU - Raschi, E.
AU - Catelli, L.
AU - Khamashta, M. A.
AU - Ichikawa, K.
AU - Tincani, A.
AU - Balestrieri, G.
AU - Meroni, P. L.
PY - 1997
Y1 - 1997
N2 - Problem: To investigate the role of the plasma cofactor for antiphospholipid antibodies in antibody binding to endothelial cells. Method of Study: a) Evaluation of endothelial cell binding of polyclonal and monoclonal anti-β2 glycoprotein I antibodies; and b) study of the effects of antibody binding: adhesion molecule expression. leukocyte adhesion, and interleukin-1β secretion. Results: Anti-β2 glycoprotein I antibodies bind endothelial cell monolayers in vitro by reacting with the cofactor adhered to the cell membranes. Antibody binding induces an upregulation of adhesion molecules, favours leukocyte adherence, and increases interleukin-1β secretion. Interleukin-1β plays an active role to mediate adhesion molecule expression through an autocrine loop, as shown by the inhibitory effect of interleukin 1 receptor antagonist. Conclusions: Antiphospholipid antibodies do react with endothelium through the cofactor adhered to their cell membranes and induces a pro-adhesive cell phenotype.
AB - Problem: To investigate the role of the plasma cofactor for antiphospholipid antibodies in antibody binding to endothelial cells. Method of Study: a) Evaluation of endothelial cell binding of polyclonal and monoclonal anti-β2 glycoprotein I antibodies; and b) study of the effects of antibody binding: adhesion molecule expression. leukocyte adhesion, and interleukin-1β secretion. Results: Anti-β2 glycoprotein I antibodies bind endothelial cell monolayers in vitro by reacting with the cofactor adhered to the cell membranes. Antibody binding induces an upregulation of adhesion molecules, favours leukocyte adherence, and increases interleukin-1β secretion. Interleukin-1β plays an active role to mediate adhesion molecule expression through an autocrine loop, as shown by the inhibitory effect of interleukin 1 receptor antagonist. Conclusions: Antiphospholipid antibodies do react with endothelium through the cofactor adhered to their cell membranes and induces a pro-adhesive cell phenotype.
KW - β2 glycoprotein I
KW - Adhesion molecules
KW - Antiphospholipid antibodies
KW - Cytokines
KW - Endothelial cells
KW - Thrombosis
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UR - http://www.scopus.com/inward/citedby.url?scp=0030880411&partnerID=8YFLogxK
M3 - Article
C2 - 9325495
AN - SCOPUS:0030880411
VL - 38
SP - 212
EP - 217
JO - American Journal of Reproductive Immunology and Microbiology
JF - American Journal of Reproductive Immunology and Microbiology
SN - 1046-7408
IS - 3
ER -