Endothelial cells express the interleukin-1 receptor type I

Diana Boraschi, Alessandro Rambaldi, Antonio Sica, Paolo Ghiara, Francesco Colotta, Ji Ming Wang, Marco De Ross, Carla Zoia, Giuseppe Remuzzi, Federico Bussolino, Giuseppe Scapigliati, Antonella Stoppacciaro, Luigi Ruco, Aldo Tagliabue, Alberto Mantovani

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-1 (IL-1) profoundly affects a number of functions of vascular cells. Two distinct IL-1 receptors (IL-1R) are expressed on different cell types: the 80 Kd IL-1R, on T cells and fibroblasts, and the 68 Kd IL-IRII on B cells and myelomonocytic cells. The presence and functionality of IL-1R on vascular cells has been investigated by using polyomatransformed mouse endothelial cell (EC) lines (sEnd.1 and tEnd.1). These cells expressed specific and saturable binding sites for IL-1 (1,273 sites per cell with kd 9.6 × 10-11 mol/L for sEnd.1, and 771 sites per cell with kd 8.5 × 10-11 mol/L for tEnd.1, with radioiodinated IL-1α as ligand). Binding of IL-1 was also evident at single cell level by autoradiography. By cross-linking studies, the molecular weight of the IL-1 binding protein on EC was approximately 80 Kd. This was confirmed by the presence in EC of mRNA for the 80 Kd IL-1RJ. The IL-IRI on EC was apparently functional, since EC responded to IL-1 with IL-6 mRNA expression and IL-6 bioactivity production. These results were extended to human EC and vascular smooth muscle cells, which were also found to express mRNA for IL-1RI.

Original languageEnglish
Pages (from-to)1262-1267
Number of pages6
JournalBlood
Volume78
Issue number5
Publication statusPublished - Sep 1 1991

ASJC Scopus subject areas

  • Hematology

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