Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype

Veronica Tisato, Giorgio Zauli, Rebecca Voltan, Sergio Gianesini, Maria Grazia Iasio, Ilaria Volpi, Guido Fiorentini, Paolo Zamboni, Paola Secchiero

Research output: Contribution to journalArticle

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Abstract

Background: The inflammatory properties of vein endothelium in relation to chronic venous disease (CVD) have been poorly investigated. Therefore, new insights on the characteristics of large vein endothelium would increase our knowledge of large vessel physiopathology. Methodology/Principal Findings: Surgical specimens of veins were obtained from the tertiary venous network (R3) and/or saphenous vein (SF) of patients affected by CVD and from control individuals. Highly purified venous endothelial cell (VEC) cultures obtained from CVD patients were characterized for morphological, phenotypic and functional properties compared to control VEC. An increase of CD31/PECAM-1, CD146 and ICAM-1 surface levels was documented at flow cytometry in pathological VEC with respect to normal controls. Of note, the strongest expression of these pro-inflammatory markers was observed in VEC obtained from patients with more advanced disease. Similarly, spontaneous cell proliferation and resistance to starvation was higher in pathological than in normal VEC, while the migratory response of VEC showed an opposite trend, being significantly lower in VEC obtained from pathological specimens. In addition, in keeping with a higher baseline transcriptional activity of NF-kB, the release of the pro-inflammatory cytokines osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF) was higher in pathological VEC cultures with respect to control VEC. Interestingly, there was a systemic correlation to these in vitro data, as demonstrated by higher serum OPG and VEGF levels in CVD patients with respect to normal healthy controls. Conclusion/Significance: Taken together, these data indicate that large vein endothelial cells obtained from CVD patients exhibit a pro-inflammatory phenotype, which might significantly contribute to systemic inflammation in CVD patients.

Original languageEnglish
Article numbere39543
JournalPLoS One
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 21 2012

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Endothelial cells
endothelial cells
Chronic Disease
Endothelial Cells
Phenotype
phenotype
Veins
Osteoprotegerin
vascular endothelial growth factors
endothelium
Cell culture
Vascular Endothelial Growth Factor A
Endothelium
cell culture
Cell Culture Techniques
saphenous vein
CD31 Antigens
transcription factor NF-kappa B
Flow cytometry
NF-kappa B

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tisato, V., Zauli, G., Voltan, R., Gianesini, S., Iasio, M. G., Volpi, I., ... Secchiero, P. (2012). Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. PLoS One, 7(6), [e39543]. https://doi.org/10.1371/journal.pone.0039543

Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. / Tisato, Veronica; Zauli, Giorgio; Voltan, Rebecca; Gianesini, Sergio; Iasio, Maria Grazia; Volpi, Ilaria; Fiorentini, Guido; Zamboni, Paolo; Secchiero, Paola.

In: PLoS One, Vol. 7, No. 6, e39543, 21.06.2012.

Research output: Contribution to journalArticle

Tisato, V, Zauli, G, Voltan, R, Gianesini, S, Iasio, MG, Volpi, I, Fiorentini, G, Zamboni, P & Secchiero, P 2012, 'Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype', PLoS One, vol. 7, no. 6, e39543. https://doi.org/10.1371/journal.pone.0039543
Tisato, Veronica ; Zauli, Giorgio ; Voltan, Rebecca ; Gianesini, Sergio ; Iasio, Maria Grazia ; Volpi, Ilaria ; Fiorentini, Guido ; Zamboni, Paolo ; Secchiero, Paola. / Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. In: PLoS One. 2012 ; Vol. 7, No. 6.
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