Endothelial colony-forming cells from patients with chronic myeloproliferative disorders lack the disease-specific molecular clonality marker

Giovanna Piaggio, Vittorio Rosti, Mirko Corselli, Francesca Bertolotti, Gaetano Bergamaschi, Sarah Pozzi, Davide Imperiale, Barbara Chiavarina, Elisa Bonetti, Francesca Novara, Mario Sessarego, Laura Villani, Anna Garuti, Margherita Massa, Riccardo Ghio, Rita Campanelli, Andrea Bacigalupo, Alessandro Pecci, Gianluca Viarengo, Orsetta ZuffardiFrancesco Frassoni, Giovanni Barosi

Research output: Contribution to journalArticle

Abstract

Two putative types of circulating endothelial progenitor cells have been recently identified in vitro: (1) endothelial colony-forming cell (ECFC) and (2) colony-forming unit-endothelial cell (CFU-EC). Only the former is now recognized to belong to endothelial lineage. We have used the ECFC and CFU-EC assays to readdress the issue of the clonal relation between endothelial progenitor cells and hematopoietic stem cells in patients with Philadelphia-positive and Philadelphia-negative chronic myeloproliferative disorders. Both ECFCs and CFUECs were cultured from peripheral blood mononuclear cells, and either BCR-ABL re-arrangement or JAK2-V617F mutation were assessed in both types of endothelial colonies. We found that ECFCs lack the disease-specific markers, which are otherwise present in CFU-ECs, thus reinforcing the concept that the latter belongs to the hematopoietic lineage, and showing that in chronic myeloproliferative disorders the cell that gives rise to circulating ECFC has a distinct origin from the cell of the hematopoietic malignant clone.

Original languageEnglish
Pages (from-to)3127-3130
Number of pages4
JournalBlood
Volume114
Issue number14
DOIs
Publication statusPublished - 2009

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ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Piaggio, G., Rosti, V., Corselli, M., Bertolotti, F., Bergamaschi, G., Pozzi, S., Imperiale, D., Chiavarina, B., Bonetti, E., Novara, F., Sessarego, M., Villani, L., Garuti, A., Massa, M., Ghio, R., Campanelli, R., Bacigalupo, A., Pecci, A., Viarengo, G., ... Barosi, G. (2009). Endothelial colony-forming cells from patients with chronic myeloproliferative disorders lack the disease-specific molecular clonality marker. Blood, 114(14), 3127-3130. https://doi.org/10.1182/blood-2008-12-190991