Endothelial G protein-coupled receptor kinase 2 regulates vascular homeostasis through the control of free radical oxygen species

Michele Ciccarelli, Daniela Sorriento, Antonietta Franco, Anna Fusco, Carmine Del Giudice, Roberto Annunziata, Ersilia Cipolletta, Maria Gaia Monti, Gerald W. Dorn, Bruno Trimarco, Guido Iaccarino

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

OBJECTIVE - : The role of endothelial G protein-coupled receptor kinase 2 (GRK2) was investigated in mice with selective deletion of the kinase in the endothelium (Tie2-CRE/GRK2). APPROACH AND RESULTS - : Aortas from Tie2-CRE/GRK2 presented functional and structural alterations as compared with control GRK2 mice. In particular, vasoconstriction was blunted to different agonists, and collagen and elastic rearrangement and macrophage infiltration were observed. In primary cultured endothelial cells deficient for GRK2, mitochondrial reactive oxygen species was increased, leading to expression of cytokines. Chronic treatment with a reactive oxygen species scavenger in mice corrected the vascular phenotype by recovering vasoconstriction, structural abnormalities, and reducing macrophage infiltration. CONCLUSIONS - : These results demonstrate that GRK2 removal compromises vascular phenotype and integrity by increasing endothelial reactive oxygen species production.

Original languageEnglish
Pages (from-to)2415-2424
Number of pages10
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume33
Issue number10
DOIs
Publication statusPublished - Oct 2013

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G-Protein-Coupled Receptor Kinase 2
Free Radicals
Blood Vessels
Homeostasis
Oxygen
Reactive Oxygen Species
Vasoconstriction
Macrophages
Phenotype
Endothelium
Aorta
Cultured Cells
Phosphotransferases
Collagen
Endothelial Cells
Cytokines

Keywords

  • endothelial cells
  • G protein-coupled receptor kinases
  • mitochondria
  • vascular medicine

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

Endothelial G protein-coupled receptor kinase 2 regulates vascular homeostasis through the control of free radical oxygen species. / Ciccarelli, Michele; Sorriento, Daniela; Franco, Antonietta; Fusco, Anna; Del Giudice, Carmine; Annunziata, Roberto; Cipolletta, Ersilia; Monti, Maria Gaia; Dorn, Gerald W.; Trimarco, Bruno; Iaccarino, Guido.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 33, No. 10, 10.2013, p. 2415-2424.

Research output: Contribution to journalArticle

Ciccarelli, M, Sorriento, D, Franco, A, Fusco, A, Del Giudice, C, Annunziata, R, Cipolletta, E, Monti, MG, Dorn, GW, Trimarco, B & Iaccarino, G 2013, 'Endothelial G protein-coupled receptor kinase 2 regulates vascular homeostasis through the control of free radical oxygen species', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 33, no. 10, pp. 2415-2424. https://doi.org/10.1161/ATVBAHA.113.302262
Ciccarelli, Michele ; Sorriento, Daniela ; Franco, Antonietta ; Fusco, Anna ; Del Giudice, Carmine ; Annunziata, Roberto ; Cipolletta, Ersilia ; Monti, Maria Gaia ; Dorn, Gerald W. ; Trimarco, Bruno ; Iaccarino, Guido. / Endothelial G protein-coupled receptor kinase 2 regulates vascular homeostasis through the control of free radical oxygen species. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2013 ; Vol. 33, No. 10. pp. 2415-2424.
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