Endothelin-1 induced MCAO: Dose dependency of cerebral blood flow

Simona Nikolova, Slavianka Moyanova, Sarah Hughes, Miranda Bellyou-Camilleri, Ting Yim Lee, Robert Bartha

Research output: Contribution to journalArticlepeer-review


The purpose of this study was to characterize the magnitude and duration of cerebral blood flow (CBF) reduction in the somatosensory cortical region in a rat model of middle cerebral artery occlusion (MCAO) induced by endothelin-1 (ET1) microinjection under isoflurane anesthesia. MCAO was induced by microinjection of ET1 proximal to the MCA in 41 isoflurane-anesthetized male Sprague-Dawley rats. Three doses of ET1 were studied, 60 pmol (Group 1), 150 pmol (Group 2), and 300 pmol (Group 3). CBF was monitored for 4 h following injection using a laser Doppler probe stereotaxically inserted into the left somatosensory cortical region. Computed tomography perfusion imaging was used to verify the extent and duration of blood flow reduction in a subset of 12 animals. The magnitude and duration of blood flow reduction was variable (60-92% of baseline). The 300 pmol dose provided the greatest sustained decrease in blood flow. Evidence of tissue damage was obtained in cases where CBF decreased to

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalJournal of Neuroscience Methods
Issue number1
Publication statusPublished - Apr 30 2009


  • Cerebral blood flow
  • Endothelin
  • Histology
  • Ischemia
  • Isoflurane
  • Rat
  • Stroke model

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Endothelin-1 induced MCAO: Dose dependency of cerebral blood flow'. Together they form a unique fingerprint.

Cite this