TY - JOUR
T1 - Endothelin-1 induced MCAO
T2 - Dose dependency of cerebral blood flow
AU - Nikolova, Simona
AU - Moyanova, Slavianka
AU - Hughes, Sarah
AU - Bellyou-Camilleri, Miranda
AU - Lee, Ting Yim
AU - Bartha, Robert
PY - 2009/4/30
Y1 - 2009/4/30
N2 - The purpose of this study was to characterize the magnitude and duration of cerebral blood flow (CBF) reduction in the somatosensory cortical region in a rat model of middle cerebral artery occlusion (MCAO) induced by endothelin-1 (ET1) microinjection under isoflurane anesthesia. MCAO was induced by microinjection of ET1 proximal to the MCA in 41 isoflurane-anesthetized male Sprague-Dawley rats. Three doses of ET1 were studied, 60 pmol (Group 1), 150 pmol (Group 2), and 300 pmol (Group 3). CBF was monitored for 4 h following injection using a laser Doppler probe stereotaxically inserted into the left somatosensory cortical region. Computed tomography perfusion imaging was used to verify the extent and duration of blood flow reduction in a subset of 12 animals. The magnitude and duration of blood flow reduction was variable (60-92% of baseline). The 300 pmol dose provided the greatest sustained decrease in blood flow. Evidence of tissue damage was obtained in cases where CBF decreased to
AB - The purpose of this study was to characterize the magnitude and duration of cerebral blood flow (CBF) reduction in the somatosensory cortical region in a rat model of middle cerebral artery occlusion (MCAO) induced by endothelin-1 (ET1) microinjection under isoflurane anesthesia. MCAO was induced by microinjection of ET1 proximal to the MCA in 41 isoflurane-anesthetized male Sprague-Dawley rats. Three doses of ET1 were studied, 60 pmol (Group 1), 150 pmol (Group 2), and 300 pmol (Group 3). CBF was monitored for 4 h following injection using a laser Doppler probe stereotaxically inserted into the left somatosensory cortical region. Computed tomography perfusion imaging was used to verify the extent and duration of blood flow reduction in a subset of 12 animals. The magnitude and duration of blood flow reduction was variable (60-92% of baseline). The 300 pmol dose provided the greatest sustained decrease in blood flow. Evidence of tissue damage was obtained in cases where CBF decreased to
KW - Cerebral blood flow
KW - Endothelin
KW - Histology
KW - Ischemia
KW - Isoflurane
KW - Rat
KW - Stroke model
UR - http://www.scopus.com/inward/record.url?scp=61649084180&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61649084180&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2009.01.009
DO - 10.1016/j.jneumeth.2009.01.009
M3 - Article
C2 - 19428507
AN - SCOPUS:61649084180
VL - 179
SP - 22
EP - 28
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 1
ER -