Endothelin and eicosanoid synthesis in cultured mesangial cells

Carla Zoja, Ariela Benigni, Daniela Renzi, Antonella Piccinelli, Norberto Perico, Giuseppe Remuzzi

Research output: Contribution to journalArticle

Abstract

We investigated the effect of endothelin on the generation of eicosanoids, which are known to regulate basal and stimulated mesangial cell tone. The results showed that endothelin is a potent stimulus of prostaglandin E2 (PGE2), prostacyclin (PGI2), and thromboxane A2 (TxA2) synthesis by bovine mesangial cells. Percentage increases in eicosanoid synthesis induced by endothelin (10-10 to 10-6 M), were 50 to 275% for PGE2, 28 to 168% for PGI2 and 42 to 111% for TxA2, respectively. Endothelin-induced eicosanoid synthesis in mesangial cells was concentration, but not time dependent. Aspirin (500 μM) completely prevented endothelin-induced eicosanoid synthesis. The calcium entry blocker nitrendipine (10-8 M) failed to inhibit endothelin-induced eicosanoid synthesis. These data suggest that endothelin-induced changes in renal circulation and glomerular function in normal and disease conditions may be modulated by the concomitant stimulation of mesangial eicosanoid synthesis.

Original languageEnglish
Pages (from-to)927-933
Number of pages7
JournalKidney International
Volume37
Issue number3
Publication statusPublished - Mar 1990

ASJC Scopus subject areas

  • Nephrology

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