Endothelin and eicosanoid synthesis in cultured mesangial cells

Carla Zoja, Ariela Benigni, Daniela Renzi, Antonella Piccinelli, Norberto Perico, Giuseppe Remuzzi

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the effect of endothelin on the generation of eicosanoids, which are known to regulate basal and stimulated mesangial cell tone. The results showed that endothelin is a potent stimulus of prostaglandin E2 (PGE2), prostacyclin (PGI2), and thromboxane A2 (TxA2) synthesis by bovine mesangial cells. Percentage increases in eicosanoid synthesis induced by endothelin (10-10 to 10-6 M), were 50 to 275% for PGE2, 28 to 168% for PGI2 and 42 to 111% for TxA2, respectively. Endothelin-induced eicosanoid synthesis in mesangial cells was concentration, but not time dependent. Aspirin (500 μM) completely prevented endothelin-induced eicosanoid synthesis. The calcium entry blocker nitrendipine (10-8 M) failed to inhibit endothelin-induced eicosanoid synthesis. These data suggest that endothelin-induced changes in renal circulation and glomerular function in normal and disease conditions may be modulated by the concomitant stimulation of mesangial eicosanoid synthesis.

Original languageEnglish
Pages (from-to)927-933
Number of pages7
JournalKidney International
Volume37
Issue number3
Publication statusPublished - Mar 1990

ASJC Scopus subject areas

  • Nephrology

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