Endothelin and sex hormones modulate the fibronectin synthesis by cultured human skin scleroderma fibroblasts

S. Soldano, P. Montagna, B. Villaggio, A. Parodi, G. Gianotti, A. Sulli, B. Seriolo, M. E. Secchi, M. Cutolo

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: To evaluate the influence of endothelin-1 (ET- 1) and sex hormones on cell proliferation and extracellular matrix (ECM) synthesis (ie, fibronectin, laminin) by cultured normal and scleroderma (SSc) human skin fibroblasts (FBs). Methods: Primary cultures of FBs were treated with ET-1 and sex hormones (17β-oestradiol or testosterone) for 24 h. Cell growth was analysed by methiltetrazolium salt test, ECM synthesis was evaluated by immunocytochemistry and western blot, both at 24 h. Results: In normal FBs, ET-1 and 17β-oestradiol, as well as their combination, increased cell growth (p

Original languageEnglish
Pages (from-to)599-602
Number of pages4
JournalAnnals of the Rheumatic Diseases
Volume68
Issue number4
DOIs
Publication statusPublished - Apr 2009

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Endothelins
Gonadal Steroid Hormones
Fibroblasts
Fibronectins
Skin
Cell growth
Extracellular Matrix
Estradiol
Cell proliferation
Laminin
Endothelin-1
Growth
Cell culture
Testosterone
Salts
Western Blotting
Immunohistochemistry
Cell Proliferation

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Allergy

Cite this

Endothelin and sex hormones modulate the fibronectin synthesis by cultured human skin scleroderma fibroblasts. / Soldano, S.; Montagna, P.; Villaggio, B.; Parodi, A.; Gianotti, G.; Sulli, A.; Seriolo, B.; Secchi, M. E.; Cutolo, M.

In: Annals of the Rheumatic Diseases, Vol. 68, No. 4, 04.2009, p. 599-602.

Research output: Contribution to journalArticle

Soldano, S, Montagna, P, Villaggio, B, Parodi, A, Gianotti, G, Sulli, A, Seriolo, B, Secchi, ME & Cutolo, M 2009, 'Endothelin and sex hormones modulate the fibronectin synthesis by cultured human skin scleroderma fibroblasts', Annals of the Rheumatic Diseases, vol. 68, no. 4, pp. 599-602. https://doi.org/10.1136/ard.2008.097378
Soldano, S. ; Montagna, P. ; Villaggio, B. ; Parodi, A. ; Gianotti, G. ; Sulli, A. ; Seriolo, B. ; Secchi, M. E. ; Cutolo, M. / Endothelin and sex hormones modulate the fibronectin synthesis by cultured human skin scleroderma fibroblasts. In: Annals of the Rheumatic Diseases. 2009 ; Vol. 68, No. 4. pp. 599-602.
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