TY - JOUR
T1 - Endotoxin requirement for macrophage activation by lymphokines in a rapid microcytotoxicity assay
AU - Taramelli, D.
AU - Holden, H. T.
AU - Varesio, L.
PY - 1980
Y1 - 1980
N2 - A short term microcytotoxicity assay is described for studying the activation of MØ by lymphokines. Proteose-peptone-induced macrophages were purified by adherence in flat-bottomed microtiter plates and then activated by MAF-containing supernatants for 18 h. 51Cr-labeled humor target cells were added for an additional 18 h, and then the supernatants were harvested and the % isotope release quantitated. When endotoxin-free medium and FBS were used, we found that small amounts of LPS were absolutely required for macrophage activation in this assay. The advantages of this technique included (a) good reproducibility, (b) the requirement for small number of MØ, and (c) the potential of standardizing the assay and thereby testing a large number of samples. Moreover, this assay may have particular value for investigations of MØ activation by exogenous stimuli, since MØ that were not pretreated with activating agents did not exhibit cytotoxicity.
AB - A short term microcytotoxicity assay is described for studying the activation of MØ by lymphokines. Proteose-peptone-induced macrophages were purified by adherence in flat-bottomed microtiter plates and then activated by MAF-containing supernatants for 18 h. 51Cr-labeled humor target cells were added for an additional 18 h, and then the supernatants were harvested and the % isotope release quantitated. When endotoxin-free medium and FBS were used, we found that small amounts of LPS were absolutely required for macrophage activation in this assay. The advantages of this technique included (a) good reproducibility, (b) the requirement for small number of MØ, and (c) the potential of standardizing the assay and thereby testing a large number of samples. Moreover, this assay may have particular value for investigations of MØ activation by exogenous stimuli, since MØ that were not pretreated with activating agents did not exhibit cytotoxicity.
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U2 - 10.1016/0022-1759(80)90309-9
DO - 10.1016/0022-1759(80)90309-9
M3 - Article
C2 - 7005345
AN - SCOPUS:0019124717
VL - 37
SP - 225
EP - 232
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 3-4
ER -