Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes

Alfredo Miccheli, Alberta Tomassini, Giorgio Capuani, Maria Enrica Di Cocco, Elisabetta Sartori, Laura Falasca, Laura Conti Devirgiliis, Cesare Manetti, Filippo Conti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We studied the effect of continuous medium flow on the viability and structural organization of hepatocytes high density entrapped in alginate gel beads in the first few hours after isolation. The metabolic energy status of the entrapped cells, monitored in vivo by 31P NMR spectroscopy, was stable during the experimental time and a physiological redox ratio was reached after the first three hours of culture. The morphological analysis revealed that the entrapped hepatocytes placed in a fixed-bed bioreactor under continuous flow showed a polyhedrical shape with numerous microvilli on cell surface and reconstituted tight junctions as well as bile canalicular structures, closely resembling those present in the liver. These results suggest that continuous flow allows the culture of hepatocytes at very high cell density within a matrix without loss of viability and accelerates cellular tissue reconstruction at very short times after isolation. This type of culture could represent a very useful model for physiological and toxicological studies as well as a promising approach toward the development of a bioartificial hybrid support device in acute liver failure.

Original languageEnglish
Pages (from-to)219-228
Number of pages10
JournalCytotechnology
Volume32
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Energy Metabolism
Hepatocytes
Adhesion
Gels
Liver
Acute Liver Failure
Tight Junctions
Alginate
Bioreactors
Microvilli
Bile
Toxicology
Nuclear magnetic resonance spectroscopy
Oxidation-Reduction
Magnetic Resonance Spectroscopy
Cell Count
Tissue
Equipment and Supplies
alginic acid

Keywords

  • Alginate
  • Bioreactor
  • Energy metabolism
  • Hepatocytes
  • Microscopy
  • NMR

ASJC Scopus subject areas

  • Biotechnology
  • Cell Biology
  • Clinical Biochemistry

Cite this

Miccheli, A., Tomassini, A., Capuani, G., Di Cocco, M. E., Sartori, E., Falasca, L., ... Conti, F. (2000). Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes. Cytotechnology, 32(3), 219-228. https://doi.org/10.1023/A:1008134005529

Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes. / Miccheli, Alfredo; Tomassini, Alberta; Capuani, Giorgio; Di Cocco, Maria Enrica; Sartori, Elisabetta; Falasca, Laura; Devirgiliis, Laura Conti; Manetti, Cesare; Conti, Filippo.

In: Cytotechnology, Vol. 32, No. 3, 2000, p. 219-228.

Research output: Contribution to journalArticle

Miccheli, A, Tomassini, A, Capuani, G, Di Cocco, ME, Sartori, E, Falasca, L, Devirgiliis, LC, Manetti, C & Conti, F 2000, 'Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes', Cytotechnology, vol. 32, no. 3, pp. 219-228. https://doi.org/10.1023/A:1008134005529
Miccheli, Alfredo ; Tomassini, Alberta ; Capuani, Giorgio ; Di Cocco, Maria Enrica ; Sartori, Elisabetta ; Falasca, Laura ; Devirgiliis, Laura Conti ; Manetti, Cesare ; Conti, Filippo. / Energy metabolism and re-establishment of intercellular adhesion complexes of gel entrapped hepatocytes. In: Cytotechnology. 2000 ; Vol. 32, No. 3. pp. 219-228.
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