Energy metabolism inhibition impairs amyloid precursor protein secretion from Alzheimer's fibroblasts

Laura Gasparini, Luisa Benussi, Angelo Bianchetti, Giuliano Binetti, Daniela Curti, Stefano Govoni, Stefania Moraschi, Marco Racchi, Marco Trabucchi

Research output: Contribution to journalArticlepeer-review

Abstract

The present study investigates the influence of aglycemia and odiUm azide (a Cytochrome c Oxidase inhibitor) on sAPP secretion from skin fibtroblasts derived from sporadic AD patients and control subjects. Aglycemia reduced sAPP release in the medium of both AD and control fibroblasts to a similar extent after 2 h incubation. Treatment for 2 h with increasing azide concentrations (1 p.M-100 mM) under glucose deprivation did not significantly affect sAPP secretion from control fibroblasts, but was able to significantly inhibit sAPP secretion from AD fibroblasts (maximal inhibition 51%). The failure of antioxidants like glutathione (GSH) or N- acetylcysteine (NAC) to antagonize the azide effect on AD fibroblasts and lipoperoxidation data seemed to rule out the possibility that oxidative stress could mediate the sodium azide effect on sAPP release from AD fibroblasts.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalNeuroscience Letters
Volume263
Issue number2-3
DOIs
Publication statusPublished - Mar 26 1999

Keywords

  • Alzheimer's disease
  • Amyloid precursor protein
  • Energy metabolism
  • Human fibroblasts
  • Sodium azide

ASJC Scopus subject areas

  • Neuroscience(all)

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