Energy metabolism of adriamycin-sensitive and -resistant Ehrlich ascites tumor cells

Stefania Miccadei, Maurizio Fanciulli, Tiziana Bruno, Marco G. Paggi, Aristide Floridi

Research output: Contribution to journalArticle

Abstract

Respiration, glycolysis, utilization of carbon from 14C-labeled glucose and the activities of some regulatory enzymes of the Krebs cycle and glycolysis in adriamycin-sensitive (EH-WT) and -resistant (EH-ADR) Ehrlich ascites tumor cells have been investigated. The following summarizes the results: 1. Compared with wild-type cells, EH-ADR cells exhibited an enhanced rate of oxygen consumption as well as of ATP production (2-fold). 2. When the cells were supplied with glucose as the only added energy source, the aerobic lactate production was 30% higher in EH-ADR cells. However, in spite of the enhanced glycolysis, 50% of total cell ATP was still supplied by oxidative phosphorylation, whereas in EH-WT cells 65% of ATP was derived from glycolysis. 3. The activities of the regulatory enzymes were remarkably more elevated in EH-ADR cells. 4. The amount of glucose carbon atoms metabolized through the Krebs cycle and pentose phosphate pathway in EH-ADR cells was significantly higher than in EH-WT cells. 5. These differences confirmed a modified energy metabolism in resistant cells and reflected metabolic adaptations associated with the development of multidrug resistance.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalOncology Research
Volume8
Issue number1
Publication statusPublished - 1996

Keywords

  • adriamycin
  • drug resistance
  • Ehrlich tumor cells
  • energy metabolism

ASJC Scopus subject areas

  • Cancer Research

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