Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells

Paola Secchiero, Davide Zella, Sabrina Curreli, Prisco Mirandola, Silvano Capitani, Robert C. Gallo, Giorgio Zauli

Research output: Contribution to journalArticle

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Abstract

Optimal CD4+ T cell activation requires the cooperation of multiple signaling pathways coupled to the TCR-CD3 complex and to the CD28 costimulatory molecule. In this study, we have investigated the expression of surface CXC chemokine receptor 4 (CXCR4) in enriched populations of CD4+ T PBL, stimulated with anti-CD3 and anti-CD28 mAbs, immobilized on plastic. Anti-CD3 alone induced a progressive down-regulation of surface CXCR4, accompanied by a significant decline in the entry of the HXB2 T cell line- tropic (X4-tropic) HIV-1 clone in CD4+ T cells. Of note, this effect was strictly dependent on the presence in culture of CD14+ monocytes. On the other hand, anti-CD28 alone induced a small but reproducible increase in the expression of surface CXCR4 as well as in the entry of HXB2 HIV-1 clone in resting CD4+ T cells. When the two mAbs were used in combination, anti-CD28 potently synergized with anti-CD3 in inducing the expression of CD69 activation marker and stimulating the proliferation of CD4+ T cells. On the other hand, anti-CD28 counteracted the CXCR4 down-modulation induced by anti- CD3. The latter effect was particularly evident when anti-CD28 was associated to suboptimal concentrations of anti-CD3. Because CXCR4 is the major coreceptor for the highly cytopathic X4-tropic HIV-1 strains, which preferentially replicate in proliferating CD4+ T cells, the ability of anti- CD28 to up-regulate the surface expression of CXCR4 in both resting and activated CD4+ T cells provides one relevant mechanism for the progression of HIV-1 disease.

Original languageEnglish
Pages (from-to)4018-4024
Number of pages7
JournalJournal of Immunology
Volume164
Issue number8
Publication statusPublished - Apr 15 2000

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CXCR4 Receptors
T-Lymphocytes
HIV-1
Clone Cells
T-Cell Antigen Receptor-CD3 Complex
Plastics
Monocytes
Up-Regulation
Down-Regulation
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Secchiero, P., Zella, D., Curreli, S., Mirandola, P., Capitani, S., Gallo, R. C., & Zauli, G. (2000). Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells. Journal of Immunology, 164(8), 4018-4024.

Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells. / Secchiero, Paola; Zella, Davide; Curreli, Sabrina; Mirandola, Prisco; Capitani, Silvano; Gallo, Robert C.; Zauli, Giorgio.

In: Journal of Immunology, Vol. 164, No. 8, 15.04.2000, p. 4018-4024.

Research output: Contribution to journalArticle

Secchiero, P, Zella, D, Curreli, S, Mirandola, P, Capitani, S, Gallo, RC & Zauli, G 2000, 'Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells', Journal of Immunology, vol. 164, no. 8, pp. 4018-4024.
Secchiero P, Zella D, Curreli S, Mirandola P, Capitani S, Gallo RC et al. Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells. Journal of Immunology. 2000 Apr 15;164(8):4018-4024.
Secchiero, Paola ; Zella, Davide ; Curreli, Sabrina ; Mirandola, Prisco ; Capitani, Silvano ; Gallo, Robert C. ; Zauli, Giorgio. / Engagement of CD28 modulates CXC chemokine receptor 4 surface expression in both resting and CD3-stimulated CD4+ T cells. In: Journal of Immunology. 2000 ; Vol. 164, No. 8. pp. 4018-4024.
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