Engineering complex muscle-tissue interfaces through microfabrication

Chiara Arrigoni, Dalila Petta, Simone Bersini, Vladimir Mironov, Christian Candrian, Matteo Moretti

Research output: Contribution to journalReview article

Abstract

Skeletal muscle is a tissue with a complex and hierarchical architecture that influences its functional properties. In order to exert its contractile function, muscle tissue is connected to neural, vascular and connective compartments, comprising finely structured interfaces which are orchestrated by multiple signalling pathways. Pathological conditions such as dystrophies and trauma, or physiological situations such as exercise and aging, modify the architectural organization of these structures, hence affecting muscle functionality. To overcome current limitations of in vivo and standard in vitro models, microfluidics and biofabrication techniques have been applied to better reproduce the microarchitecture and physicochemical environment of human skeletal muscle tissue. In the present review, we aim to critically discuss the role of those techniques, taken individually or in combination, in the generation of models that mimic the complex interfaces between muscle tissue and neural/vascular/tendon compartments. The exploitation of either microfluidics or biofabrication to model different muscle interfaces has led to the development of constructs with an improved spatial organization, thus presenting a better functionality as compared to standard models. However, the achievement of models replicating muscle-tissue interfaces with adequate architecture, presence of fundamental proteins and recapitulation of signalling pathways is still far from being achieved. Increased integration between microfluidics and biofabrication, providing the possibility to pattern cells in predetermined structures with higher resolution, will help to reproduce the hierarchical and heterogeneous structure of skeletal muscle interfaces. Such strategies will further improve the functionality of these techniques, providing a key contribution towards the study of skeletal muscle functions in physiology and pathology.

Original languageEnglish
Article number032004
JournalBiofabrication
Volume11
Issue number3
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Microtechnology
Microfabrication
Muscle
Tissue
Muscles
Microfluidics
Skeletal Muscle
Blood Vessels
Tendons
Physiology
Pathology
Wounds and Injuries
Aging of materials
Proteins

Keywords

  • biofabrication
  • microfluidics
  • myotendinous junction
  • neuromuscular junction
  • vascularized skeletal muscle tissue

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Arrigoni, C., Petta, D., Bersini, S., Mironov, V., Candrian, C., & Moretti, M. (2019). Engineering complex muscle-tissue interfaces through microfabrication. Biofabrication, 11(3), [032004]. https://doi.org/10.1088/1758-5090/ab1e7c

Engineering complex muscle-tissue interfaces through microfabrication. / Arrigoni, Chiara; Petta, Dalila; Bersini, Simone; Mironov, Vladimir; Candrian, Christian; Moretti, Matteo.

In: Biofabrication, Vol. 11, No. 3, 032004, 01.01.2019.

Research output: Contribution to journalReview article

Arrigoni, C, Petta, D, Bersini, S, Mironov, V, Candrian, C & Moretti, M 2019, 'Engineering complex muscle-tissue interfaces through microfabrication', Biofabrication, vol. 11, no. 3, 032004. https://doi.org/10.1088/1758-5090/ab1e7c
Arrigoni, Chiara ; Petta, Dalila ; Bersini, Simone ; Mironov, Vladimir ; Candrian, Christian ; Moretti, Matteo. / Engineering complex muscle-tissue interfaces through microfabrication. In: Biofabrication. 2019 ; Vol. 11, No. 3.
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