Engineering human cell-based, functionally integrated osteochondral grafts by biological bonding of engineered cartilage tissues to bony scaffolds

Celeste Scotti, Dieter Wirz, Francine Wolf, Dirk J. Schaefer, Vivienne Bürgin, Alma U. Daniels, Victor Valderrabano, Christian Candrian, Marcel Jakob, Ivan Martin, Andrea Barbero

Research output: Contribution to journalArticle

Abstract

In this study, we aimed at developing and validating a technique for the engineering of osteochondral grafts based on the biological bonding of a chondral layer with a bony scaffold by cell-laid extracellular matrix. Osteochondral composites were generated by combining collagen-based matrices (Chondro-Gide®) containing human chondrocytes with devitalized spongiosa cylinders (Tutobone®) using a fibrin gel (Tisseel®). We demonstrate that separate pre-culture of the chondral layer for 3 days prior to the generation of the composite allows for (i) more efficient cartilaginous matrix accumulation than no pre-culture, as assessed histologically and biochemically, and (ii) superior biological bonding to the bony scaffold than 14 days of pre-culture, as assessed using a peel-off mechanical test, developed to measure integration of bilayered materials. The presence of the bony scaffold induced an upregulation in the infiltrated cells of the osteoblast-related gene bone sialoprotein, indicative of the establishment of a gradient of cell phenotypes, but did not affect per se the quality of the cartilaginous matrix in the chondral layer. The described strategy to generate osteochondral plugs is simple to be implemented and - since it is based on clinically compliant cells and materials - is amenable to be readily tested in the clinic.

Original languageEnglish
Pages (from-to)2252-2259
Number of pages8
JournalBiomaterials
Volume31
Issue number8
DOIs
Publication statusPublished - Mar 2010

Keywords

  • Biomimetic material
  • Cartilage tissue engineering
  • Chondrocyte
  • Integration
  • Interface

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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  • Cite this

    Scotti, C., Wirz, D., Wolf, F., Schaefer, D. J., Bürgin, V., Daniels, A. U., Valderrabano, V., Candrian, C., Jakob, M., Martin, I., & Barbero, A. (2010). Engineering human cell-based, functionally integrated osteochondral grafts by biological bonding of engineered cartilage tissues to bony scaffolds. Biomaterials, 31(8), 2252-2259. https://doi.org/10.1016/j.biomaterials.2009.11.110