Engineering human monoclonal antibody fragments

a recombinant enzyme-linked Fab.

R. Burioni, P. Plaisant, M. L. Riccio, G. M. Rossolini, R. Santangelo, A. Vannini, G. Satta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A new plasmid vector, pCRP, allowing the expression of human recombinant monoclonal antibody Fab fragments fused with a bacterial acid phosphatase has been constructed. pCRP can accept heavy- and light-chain cDNAs cloned from combinatorial antibody libraries displayed on filamentous phages with the pCombIII system and is able to direct expression to soluble Fabs in which the carboxy-terminus of the heavy chain is fused to the amino-terminus of the mature PhoN nonspecific acid phosphatase of Providencia stuartii. Using the pCRP vector, we expressed two different human recombinant Fabs cloned from combinatorial libraries (one anti-tetenus toxoid and the other anti-HIV-1 gp120) fused with the acid phosphatase. In both cases chimeric antibodies were obtained which retained the antigen-binding ability and the enzymatic activity. Similar Fab-enzyme fusions can be successfully used, even unpurified, in enzyme immunoassays.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalNew Microbiologica
Volume18
Issue number2
Publication statusPublished - Apr 1995

Fingerprint

Immunoglobulin Fragments
Human Engineering
Acid Phosphatase
Monoclonal Antibodies
Enzymes
Providencia
Toxoids
Immunoglobulin Fab Fragments
Aptitude
Antibodies
Immunoenzyme Techniques
Bacteriophages
HIV-1
Plasmids
Complementary DNA
Light
Antigens

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Burioni, R., Plaisant, P., Riccio, M. L., Rossolini, G. M., Santangelo, R., Vannini, A., & Satta, G. (1995). Engineering human monoclonal antibody fragments: a recombinant enzyme-linked Fab. New Microbiologica, 18(2), 127-133.

Engineering human monoclonal antibody fragments : a recombinant enzyme-linked Fab. / Burioni, R.; Plaisant, P.; Riccio, M. L.; Rossolini, G. M.; Santangelo, R.; Vannini, A.; Satta, G.

In: New Microbiologica, Vol. 18, No. 2, 04.1995, p. 127-133.

Research output: Contribution to journalArticle

Burioni, R, Plaisant, P, Riccio, ML, Rossolini, GM, Santangelo, R, Vannini, A & Satta, G 1995, 'Engineering human monoclonal antibody fragments: a recombinant enzyme-linked Fab.', New Microbiologica, vol. 18, no. 2, pp. 127-133.
Burioni R, Plaisant P, Riccio ML, Rossolini GM, Santangelo R, Vannini A et al. Engineering human monoclonal antibody fragments: a recombinant enzyme-linked Fab. New Microbiologica. 1995 Apr;18(2):127-133.
Burioni, R. ; Plaisant, P. ; Riccio, M. L. ; Rossolini, G. M. ; Santangelo, R. ; Vannini, A. ; Satta, G. / Engineering human monoclonal antibody fragments : a recombinant enzyme-linked Fab. In: New Microbiologica. 1995 ; Vol. 18, No. 2. pp. 127-133.
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