Abstract
A new plasmid vector, pCRP, allowing the expression of human recombinant monoclonal antibody Fab fragments fused with a bacterial acid phosphatase has been constructed. pCRP can accept heavy- and light-chain cDNAs cloned from combinatorial antibody libraries displayed on filamentous phages with the pCombIII system and is able to direct expression to soluble Fabs in which the carboxy-terminus of the heavy chain is fused to the amino-terminus of the mature PhoN nonspecific acid phosphatase of Providencia stuartii. Using the pCRP vector, we expressed two different human recombinant Fabs cloned from combinatorial libraries (one anti-tetenus toxoid and the other anti-HIV-1 gp120) fused with the acid phosphatase. In both cases chimeric antibodies were obtained which retained the antigen-binding ability and the enzymatic activity. Similar Fab-enzyme fusions can be successfully used, even unpurified, in enzyme immunoassays.
| Original language | English |
|---|---|
| Pages (from-to) | 127-133 |
| Number of pages | 7 |
| Journal | New Microbiologica |
| Volume | 18 |
| Issue number | 2 |
| Publication status | Published - Apr 1995 |
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ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Engineering human monoclonal antibody fragments : a recombinant enzyme-linked Fab. / Burioni, R.; Plaisant, P.; Riccio, M. L.; Rossolini, G. M.; Santangelo, R.; Vannini, A.; Satta, G.
In: New Microbiologica, Vol. 18, No. 2, 04.1995, p. 127-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Engineering human monoclonal antibody fragments
T2 - a recombinant enzyme-linked Fab.
AU - Burioni, R.
AU - Plaisant, P.
AU - Riccio, M. L.
AU - Rossolini, G. M.
AU - Santangelo, R.
AU - Vannini, A.
AU - Satta, G.
PY - 1995/4
Y1 - 1995/4
N2 - A new plasmid vector, pCRP, allowing the expression of human recombinant monoclonal antibody Fab fragments fused with a bacterial acid phosphatase has been constructed. pCRP can accept heavy- and light-chain cDNAs cloned from combinatorial antibody libraries displayed on filamentous phages with the pCombIII system and is able to direct expression to soluble Fabs in which the carboxy-terminus of the heavy chain is fused to the amino-terminus of the mature PhoN nonspecific acid phosphatase of Providencia stuartii. Using the pCRP vector, we expressed two different human recombinant Fabs cloned from combinatorial libraries (one anti-tetenus toxoid and the other anti-HIV-1 gp120) fused with the acid phosphatase. In both cases chimeric antibodies were obtained which retained the antigen-binding ability and the enzymatic activity. Similar Fab-enzyme fusions can be successfully used, even unpurified, in enzyme immunoassays.
AB - A new plasmid vector, pCRP, allowing the expression of human recombinant monoclonal antibody Fab fragments fused with a bacterial acid phosphatase has been constructed. pCRP can accept heavy- and light-chain cDNAs cloned from combinatorial antibody libraries displayed on filamentous phages with the pCombIII system and is able to direct expression to soluble Fabs in which the carboxy-terminus of the heavy chain is fused to the amino-terminus of the mature PhoN nonspecific acid phosphatase of Providencia stuartii. Using the pCRP vector, we expressed two different human recombinant Fabs cloned from combinatorial libraries (one anti-tetenus toxoid and the other anti-HIV-1 gp120) fused with the acid phosphatase. In both cases chimeric antibodies were obtained which retained the antigen-binding ability and the enzymatic activity. Similar Fab-enzyme fusions can be successfully used, even unpurified, in enzyme immunoassays.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029281771&partnerID=8YFLogxK
M3 - Article
VL - 18
SP - 127
EP - 133
JO - New Microbiologica
JF - New Microbiologica
SN - 1121-7138
IS - 2
ER -