Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats

Luzzi Sabino, Crovace Alberto Maria, Lacitignola Luca, Valentini Valerio, Francioso Edda, Rossi Giacomo, Invernici Gloria, Galzio Renato Juan, Crovace Antonio

Research output: Contribution to journalArticle

Abstract

Background: Proof of the efficacy and safety of a xenogeneic mesenchymal stem cell (MSCs) transplant for spinal cord injury (SCI) may theoretically widen the spectrum of possible grafts for neuroregeneration. Methods: Twenty rats were submitted to complete spinal cord transection. Ovine bone marrow MSCs, retrovirally transfected with red fluorescent protein and not previously induced for neuroglial differentiation, were applied in 10 study rats (MSCG). Fibrin glue was injected in 10 control rats (FGG). All rats were evaluated on a weekly basis and scored using the Basso-Beattie-Bresnahan (BBB) locomotor scale for 10 weeks, when the collected data were statistically analyzed. The spinal cords were then harvested and analyzed with light microscopy, immunohistochemistry, and immunofluorescence. Results: Ovine MSCs culture showed positivity for Nestin. MSCG had a significant and durable recovery of motor functions (P <.001). Red fluorescence was found at the injury sites in MSCG. Positivity for Nestin, tubulin βIII, NG2 glia, neuron-specific enolase, vimentin, and 200 kD neurofilament were also found at the same sites. Conclusions: Xenogeneic ovine bone marrow MSCs proved capable of engrafting into the injured rat spinal cord. Transdifferentiation into a neuroglial phenotype was able to support partial functional recovery.

Original languageEnglish
Article number19
JournalSurgical Neurology International
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 2018
Externally publishedYes

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Spinal Cord Injuries
Mesenchymal Stromal Cells
Nestin
Sheep
Spinal Cord
Bone Marrow
Transplants
Fibrin Tissue Adhesive
Phosphopyruvate Hydratase
Recovery of Function
Vimentin
Tubulin
Fluorescence Microscopy
Neuroglia
Cell Culture Techniques
Fluorescence
Immunohistochemistry
Phenotype
Safety
Light

Keywords

  • Mesenchymal stem cells
  • neuroglial differentiation
  • neurological recovery
  • red fluorescent protein
  • spinal cord injury

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Cite this

Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats. / Sabino, Luzzi; Maria, Crovace Alberto; Luca, Lacitignola; Valerio, Valentini; Edda, Francioso; Giacomo, Rossi; Gloria, Invernici; Juan, Galzio Renato; Antonio, Crovace.

In: Surgical Neurology International, Vol. 9, No. 1, 19, 01.01.2018.

Research output: Contribution to journalArticle

Sabino, L, Maria, CA, Luca, L, Valerio, V, Edda, F, Giacomo, R, Gloria, I, Juan, GR & Antonio, C 2018, 'Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats', Surgical Neurology International, vol. 9, no. 1, 19. https://doi.org/10.4103/sni.sni_369_17
Sabino, Luzzi ; Maria, Crovace Alberto ; Luca, Lacitignola ; Valerio, Valentini ; Edda, Francioso ; Giacomo, Rossi ; Gloria, Invernici ; Juan, Galzio Renato ; Antonio, Crovace. / Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats. In: Surgical Neurology International. 2018 ; Vol. 9, No. 1.
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