Enhanced adrenal renin and aldosterone biosynthesis during sodium restriction in TGR (mREN2)27

S. Rubattu, I. Enea, D. Ganten, D. Salvatore, G. Condorelli, G. Condorelli, R. Russo, M. Romano, B. Gigante, B. Trimarco, M. Volpe

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the study was to investigate the relationships between tissue renin and the steroid production in the adrenal cortex during dietary sodium restriction in the transgenic rat (TGR) (mREN2)27. Thus the effects of a 1- wk low-sodium intake (0.04% NaCl) were studied in 5-wk-old male TGR (n = 33, systolic blood pressure = 151 ± 3 mmHg) and in 24 age- and sex-matched outbred normotensive Sprague-Dawley (SD) rats. Measurements of plasma and tissue hormones were obtained at 0, 4, and 7 days of a low sodium diet. Sodium restriction caused sustained increases of adrenal renin activity (from 28.5 ± 3.5 to 87.5 ± 4.5 ng · mg protein-1 · h-1 on day 7) and of adrenal renin mRNA (+63 ± 13 and +43 ± 7% on days 4 and 7, respectively, whereas plasma renin activity (from 3.3 ± 0.3 to 4.4 ± 0.6 ng · ml-1 · h-1) and renal renin activity (from 0.85 ± 0.25 to 0.7 ± 0.4 μg · mg protein-1 · h-1) did not change. The stimulation of the adrenal renin- angiotensin system was associated with a large increase of the aldosterone synthase cytochrome P 450 mRNA (+165 ± 35 and +184 ± 44%, on days 4 and 7) and of plasma aldosterone levels (from 125 ± 32 to 338 ± 59 μg/ml), P <0.01. In SD rats, in spite of a more consistent increase in renal and circulating renin, mineralocorticoid production did not increase significantly. These results demonstrate that the exaggerated biosynthesis of aldosterone in TGR during sodium restriction is associated with an activation of renin in the adrenal cortex but not in the kidney.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume267
Issue number4 30-4
Publication statusPublished - 1994

Keywords

  • adrenal glands
  • aldosterone
  • hypertension
  • kidney
  • renin-angiotensin system
  • transgenic rat

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Fingerprint Dive into the research topics of 'Enhanced adrenal renin and aldosterone biosynthesis during sodium restriction in TGR (mREN2)27'. Together they form a unique fingerprint.

Cite this