TY - JOUR
T1 - Enhanced and coordinated in vivo expression of inflammatory cytokines and nitric oxide synthase by chondrocytes from patients with osteoarthritis
AU - Melchiorri, Cinzia
AU - Meliconi, Riccardo
AU - Frizziero, Luigi
AU - Silvestri, Tania
AU - Pulsatelli, Lia
AU - Mazzetti, Ilaria
AU - Borzì, Rosa Maria
AU - Uguccioni, Mariagrazia
AU - Facchini, Andrea
PY - 1998
Y1 - 1998
N2 - Objective. To evaluate the sites of expression of interleukin-1β (IL- 1β), tumor necrosis factor (TNFα), and inducible nitric oxide synthase (iNOS) in patients with inflammatory and degenerative joint diseases. Methods. Cytokines and iNOS were detected by immunohistochemistry analysis of synovial and cartilage biopsy specimens obtained at knee arthroscopy in patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), osteoarthritis (OA), and traumatic knee arthritis. Cytokine and iNOS expression was quantified using computerized image analysis. Results. IL- 1β, TNFα, and iNOS were highly expressed by synovial cells (lining layer cells, infiltrating leukocytes, endothelial cells) from patients with inflammatory arthritides and significantly less by synovial cells from patients with OA and traumatic arthritis. In contrast, the latter patients showed high chondrocyte expression of cytokines and iNOS while RA and PsA patients had only minor chondrocyte positivity. In both joint compartments, IL-1β expression, TNFα expression, and iNOS expression were strongly correlated. Conclusion. The enhanced and coordinated expression of IL-1β, TNFα, and iNOS by chondrocytes strongly supports the hypothesis that chondrocytes are the major site of production of mediators of inflammation in human OA, thus playing a primary role in the pathogenesis of this disease.
AB - Objective. To evaluate the sites of expression of interleukin-1β (IL- 1β), tumor necrosis factor (TNFα), and inducible nitric oxide synthase (iNOS) in patients with inflammatory and degenerative joint diseases. Methods. Cytokines and iNOS were detected by immunohistochemistry analysis of synovial and cartilage biopsy specimens obtained at knee arthroscopy in patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), osteoarthritis (OA), and traumatic knee arthritis. Cytokine and iNOS expression was quantified using computerized image analysis. Results. IL- 1β, TNFα, and iNOS were highly expressed by synovial cells (lining layer cells, infiltrating leukocytes, endothelial cells) from patients with inflammatory arthritides and significantly less by synovial cells from patients with OA and traumatic arthritis. In contrast, the latter patients showed high chondrocyte expression of cytokines and iNOS while RA and PsA patients had only minor chondrocyte positivity. In both joint compartments, IL-1β expression, TNFα expression, and iNOS expression were strongly correlated. Conclusion. The enhanced and coordinated expression of IL-1β, TNFα, and iNOS by chondrocytes strongly supports the hypothesis that chondrocytes are the major site of production of mediators of inflammation in human OA, thus playing a primary role in the pathogenesis of this disease.
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U2 - 10.1002/1529-0131(199812)41:12<2165::AID-ART11>3.0.CO;2-O
DO - 10.1002/1529-0131(199812)41:12<2165::AID-ART11>3.0.CO;2-O
M3 - Article
C2 - 9870873
AN - SCOPUS:0032432868
VL - 41
SP - 2165
EP - 2174
JO - Arthritis care and research : the official journal of the Arthritis Health Professions Association
JF - Arthritis care and research : the official journal of the Arthritis Health Professions Association
SN - 0893-7524
IS - 12
ER -