TY - JOUR
T1 - Enhanced antisense effect of modified PNAs delivered through functional PMMA microspheres
AU - Chiarantini, Laura
AU - Cerasi, Aurora
AU - Millo, Enrico
AU - Sparnacci, Katia
AU - Laus, Michele
AU - Riccio, Massimo
AU - Santi, Spartaco
AU - Ballestri, Marco
AU - Spaccasassi, Silvia
AU - Tondelli, Luisa
PY - 2006/10/31
Y1 - 2006/10/31
N2 - Peptide nucleic acids (PNA) are very promising antisense agents, but their in vivo application is often hampered by their low bioavailability, mainly due to their limited uptake through cellular and nuclear membranes. However, PNA chemical synthesis easily allows modification with functional structures able to improve the intrinsically low permeability and great interest is arising in finding specific and efficient delivery protocols. Polymeric core-shell microspheres with anionic functional groups on the surface were tested for their ability to reversibly bind lysine modified PNA sequences, whose antisense activity against COX-2 mRNA was already demonstrated in murine macrophages.
AB - Peptide nucleic acids (PNA) are very promising antisense agents, but their in vivo application is often hampered by their low bioavailability, mainly due to their limited uptake through cellular and nuclear membranes. However, PNA chemical synthesis easily allows modification with functional structures able to improve the intrinsically low permeability and great interest is arising in finding specific and efficient delivery protocols. Polymeric core-shell microspheres with anionic functional groups on the surface were tested for their ability to reversibly bind lysine modified PNA sequences, whose antisense activity against COX-2 mRNA was already demonstrated in murine macrophages.
KW - Antisense therapy
KW - Ciclooxigenase-2 (COX-2)
KW - Core-shell microspheres
KW - Macrophages
KW - Peptide nucleic acids (PNA)
KW - PMMA microspheres
UR - http://www.scopus.com/inward/record.url?scp=33749647335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749647335&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2006.07.007
DO - 10.1016/j.ijpharm.2006.07.007
M3 - Article
C2 - 16926075
AN - SCOPUS:33749647335
VL - 324
SP - 83
EP - 91
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1
ER -