Enhanced Drug Delivery into Cell Cytosol via Glycoprotein H-Derived Peptide Conjugated Nanoemulsions

Teresa Fotticchia, Raffaele Vecchione, Pasqualina Liana Scognamiglio, Daniela Guarnieri, Vincenzo Calcagno, Concetta Di Natale, Chiara Attanasio, Maria De Gregorio, Chiara Di Cicco, Vincenzo Quagliariello, Nicola Maurea, Antonio Barbieri, Claudio Arra, Luca Raiola, Rosario V. Iaffaioli, Paolo A. Netti

Research output: Contribution to journalArticle

Abstract

The key role of nanocarriers in improving the pharmacological properties of commonly used drugs is recognized worldwide. It is also known that in the development of new effective nanocarriers the use of targeting moieties integrated on their surface is essential. Herein, we propose a nanocarrier based on an oil in water nanoemulsion coated with a membranotropic peptide derived from the glycoprotein H of Herpes simplex virus 1, known as gH625, in order to reduce endolysosomal accumulation and to enhance cytosolic localization. In addition, we show an enhanced anti-inflammatory activity of curcumin, a bioactive compound isolated from the Curcuma longa plant, when loaded into our engineered nanocarriers. This effect is a consequence of a higher uptake combined with a high curcumin preservation exerted by the active nanocapsules compared to control ones. When loaded into our nanocapsules, indeed, curcumin molecules are directly internalized into the cytosol rather than into lysosomes. Further, in order to extend the in vitro experimental setting with a more complex model and to explore the possibility to use our nanocarriers for further biological applications, we tested their performance in a 3D sprouting angiogenesis model. Finally, we show promising preliminary in vivo results by assessing the anti-inflammatory properties of the proposed nanocarrier.

Original languageEnglish
Pages (from-to)9802-9813
Number of pages12
JournalACS Nano
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 24 2017

    Fingerprint

Keywords

  • curcumin
  • drug delivery
  • gH625
  • nanocapsules
  • nanoemulsions

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Fotticchia, T., Vecchione, R., Scognamiglio, P. L., Guarnieri, D., Calcagno, V., Di Natale, C., Attanasio, C., De Gregorio, M., Di Cicco, C., Quagliariello, V., Maurea, N., Barbieri, A., Arra, C., Raiola, L., Iaffaioli, R. V., & Netti, P. A. (2017). Enhanced Drug Delivery into Cell Cytosol via Glycoprotein H-Derived Peptide Conjugated Nanoemulsions. ACS Nano, 11(10), 9802-9813. https://doi.org/10.1021/acsnano.7b03058