Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

Alberto Diaspro, Silke Krol, Barbara Campanini, Fabio Cannone, Giuseppe Chirico

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Abstract

Discovery of Green Fluorescent Protein (GFP) constituted an important improvement for living cell studies on submicron resolution allowing in vivo fluorescence labeling. We studied the photo-physical properties of single GFP molecules incorporated in a charged polyelectrolyte environment by means of single molecule spectroscopy. The fluorescence characteristics change dramatically in terms of photo-stability, lifetime and blinking behavior so that the proteins scale up to quantum dots. The reported results highlight interesting applications in the design of fluorescent markers and in the development of optical data storage architectures.

Original languageEnglish
JournalOptics Express
Volume14
Issue number21
Publication statusPublished - 2006

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Diaspro, A., Krol, S., Campanini, B., Cannone, F., & Chirico, G. (2006). Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging. Optics Express, 14(21).