Enhanced healing of diabetic wounds by topical administration of adipose tissue-derived stromal cells overexpressing stromal-derived factor-1: Biodistribution and engraftment analysis by bioluminescent imaging

Giuliana Di Rocco, Antonietta Gentile, Annalisa Antonini, Francesca Ceradini, Joseph C. Wu, Maurizio C. Capogrossi, Gabriele Toietta

Research output: Contribution to journalArticlepeer-review

Abstract

Chronic ulcers represent a major health problem in diabetic patients resulting in pain and discomfort. Conventional therapy does not guarantee adequate wound repair. In diabetes, impaired healing is partly due to poor endothelial progenitor cells mobilisation and homing, with altered levels of the chemokine stromal-derived factor-1 (SDF-1) at the wound site. Adipose tissue-associated stromal cells (AT-SCs) can provide an accessible source of progenitor cells secreting proangiogenic factors and differentiating into endothelial-like cells. We demonstrated that topical administration of AT-SCs genetically modified ex vivo to overexpress SDF-1, promotes wound healing into diabetic mice. In particular, by in vivo bioluminescent imaging analysis, we monitored biodistribution and survival after transplantation of luciferase-expressing cells. In conclusion, this study indicates the therapeutic potential of AT-SCs administration in wound healing, through cell differentiation, enhanced cellular recruitment at the wound site, and paracrine effects associated with local growth-factors production.

Original languageEnglish
Article number304562
JournalStem Cells International
DOIs
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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