Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies: Effect of antioxidant treatment

D. Ferro, M. Saliola, P. L. Meroni, G. Valesini, C. Caroselli, D. Praticó, G. A. Fitzgerald, Y. Shoenfeld, F. Violi

Research output: Contribution to journalArticle

Abstract

In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalJournal of Thrombosis and Haemostasis
Volume1
Issue number3
DOIs
Publication statusPublished - Mar 2003

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Antiphospholipid Antibodies
Thromboplastin
Monocytes
Oxidative Stress
Antioxidants
Vitamin E
Isoprostanes
Superoxides
Glycoproteins
Therapeutics
Antibodies
Ascorbic Acid
Free Radicals
Reactive Oxygen Species
Cross-Sectional Studies
Immunoglobulin G
Antigens

Keywords

  • Antiphospholipid antibodies
  • Oxidative stress
  • Tissue factor

ASJC Scopus subject areas

  • Hematology
  • Medicine(all)

Cite this

Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies : Effect of antioxidant treatment. / Ferro, D.; Saliola, M.; Meroni, P. L.; Valesini, G.; Caroselli, C.; Praticó, D.; Fitzgerald, G. A.; Shoenfeld, Y.; Violi, F.

In: Journal of Thrombosis and Haemostasis, Vol. 1, No. 3, 03.2003, p. 523-531.

Research output: Contribution to journalArticle

Ferro, D. ; Saliola, M. ; Meroni, P. L. ; Valesini, G. ; Caroselli, C. ; Praticó, D. ; Fitzgerald, G. A. ; Shoenfeld, Y. ; Violi, F. / Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies : Effect of antioxidant treatment. In: Journal of Thrombosis and Haemostasis. 2003 ; Vol. 1, No. 3. pp. 523-531.
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