Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies

Effect of antioxidant treatment

D. Ferro, M. Saliola, P. L. Meroni, G. Valesini, C. Caroselli, D. Praticó, G. A. Fitzgerald, Y. Shoenfeld, F. Violi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalJournal of Thrombosis and Haemostasis
Volume1
Issue number3
DOIs
Publication statusPublished - Mar 2003

Fingerprint

Antiphospholipid Antibodies
Thromboplastin
Monocytes
Oxidative Stress
Antioxidants
Vitamin E
Isoprostanes
Superoxides
Glycoproteins
Therapeutics
Antibodies
Ascorbic Acid
Free Radicals
Reactive Oxygen Species
Cross-Sectional Studies
Immunoglobulin G
Antigens

Keywords

  • Antiphospholipid antibodies
  • Oxidative stress
  • Tissue factor

ASJC Scopus subject areas

  • Hematology
  • Medicine(all)

Cite this

Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies : Effect of antioxidant treatment. / Ferro, D.; Saliola, M.; Meroni, P. L.; Valesini, G.; Caroselli, C.; Praticó, D.; Fitzgerald, G. A.; Shoenfeld, Y.; Violi, F.

In: Journal of Thrombosis and Haemostasis, Vol. 1, No. 3, 03.2003, p. 523-531.

Research output: Contribution to journalArticle

Ferro, D. ; Saliola, M. ; Meroni, P. L. ; Valesini, G. ; Caroselli, C. ; Praticó, D. ; Fitzgerald, G. A. ; Shoenfeld, Y. ; Violi, F. / Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies : Effect of antioxidant treatment. In: Journal of Thrombosis and Haemostasis. 2003 ; Vol. 1, No. 3. pp. 523-531.
@article{d352789c2dcd407a9d180724773665c2,
title = "Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies: Effect of antioxidant treatment",
abstract = "In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.",
keywords = "Antiphospholipid antibodies, Oxidative stress, Tissue factor",
author = "D. Ferro and M. Saliola and Meroni, {P. L.} and G. Valesini and C. Caroselli and D. Pratic{\'o} and Fitzgerald, {G. A.} and Y. Shoenfeld and F. Violi",
year = "2003",
month = "3",
doi = "10.1046/j.1538-7836.2003.00108.x",
language = "English",
volume = "1",
pages = "523--531",
journal = "Journal of Thrombosis and Haemostasis",
issn = "1538-7933",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies

T2 - Effect of antioxidant treatment

AU - Ferro, D.

AU - Saliola, M.

AU - Meroni, P. L.

AU - Valesini, G.

AU - Caroselli, C.

AU - Praticó, D.

AU - Fitzgerald, G. A.

AU - Shoenfeld, Y.

AU - Violi, F.

PY - 2003/3

Y1 - 2003/3

N2 - In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.

AB - In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.

KW - Antiphospholipid antibodies

KW - Oxidative stress

KW - Tissue factor

UR - http://www.scopus.com/inward/record.url?scp=0642280708&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0642280708&partnerID=8YFLogxK

U2 - 10.1046/j.1538-7836.2003.00108.x

DO - 10.1046/j.1538-7836.2003.00108.x

M3 - Article

VL - 1

SP - 523

EP - 531

JO - Journal of Thrombosis and Haemostasis

JF - Journal of Thrombosis and Haemostasis

SN - 1538-7933

IS - 3

ER -