TY - JOUR
T1 - Enhanced neurogenesis during trimethyltin-induced neurodegeneration in the hippocampus of the adult rat
AU - Corvino, Valentina
AU - Geloso, Maria Concetta
AU - Cavallo, Valentina
AU - Guadagni, Enrico
AU - Passalacqua, Roberto
AU - Florenzano, Fulvio
AU - Giannetti, Stefano
AU - Molinari, Marco
AU - Michetti, Fabrizio
PY - 2005/5/30
Y1 - 2005/5/30
N2 - The occurrence of neurogenesis in the hippocampus of the adult rat during trimethyltin (TMT)-induced neurodegeneration was investigated using bromodeoxyuridine (BrdU). Fifteen days after TMT intoxication, BrdU-labeled cells were significantly more numerous in the hippocampus of treated animals, gradually decreasing towards the control value 21 days after intoxication in the dentate gyrus (DG), while in the CA3/hilus region BrdU-labeled cells were still more numerous in TMT-treated rats. In order to investigate the fate of newly-generated cells double labeling experiments using neuronal or glial markers were performed. Colocalization of the neuronal marker NeuN was detected in many BrdU-positive cells in the DG, while in the CA3/hilus region no colocalization of NeuN and BrdU could be observed. No colocalization of BrdU and the astroglial marker GFAP or the microglial marker OX-42 was detected either in the DG and or in the CA3/hilus region. The results indicate an enhancement of endogenous neurogenesis in the hippocampus during TMT-induced neurodegeneration, with the development of a subpopulation of regenerated cells into neurons in the DG, while in the CA3/hilus region the population of newly-generated cells should be regarded as undifferentiated.
AB - The occurrence of neurogenesis in the hippocampus of the adult rat during trimethyltin (TMT)-induced neurodegeneration was investigated using bromodeoxyuridine (BrdU). Fifteen days after TMT intoxication, BrdU-labeled cells were significantly more numerous in the hippocampus of treated animals, gradually decreasing towards the control value 21 days after intoxication in the dentate gyrus (DG), while in the CA3/hilus region BrdU-labeled cells were still more numerous in TMT-treated rats. In order to investigate the fate of newly-generated cells double labeling experiments using neuronal or glial markers were performed. Colocalization of the neuronal marker NeuN was detected in many BrdU-positive cells in the DG, while in the CA3/hilus region no colocalization of NeuN and BrdU could be observed. No colocalization of BrdU and the astroglial marker GFAP or the microglial marker OX-42 was detected either in the DG and or in the CA3/hilus region. The results indicate an enhancement of endogenous neurogenesis in the hippocampus during TMT-induced neurodegeneration, with the development of a subpopulation of regenerated cells into neurons in the DG, while in the CA3/hilus region the population of newly-generated cells should be regarded as undifferentiated.
KW - Bromodeoxyuridine
KW - Hippocampus
KW - Neurodegeneration
KW - Neurogenesis
KW - Trimethyltin
UR - http://www.scopus.com/inward/record.url?scp=18144377109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18144377109&partnerID=8YFLogxK
U2 - 10.1016/j.brainresbull.2005.02.031
DO - 10.1016/j.brainresbull.2005.02.031
M3 - Article
C2 - 15862918
AN - SCOPUS:18144377109
VL - 65
SP - 471
EP - 477
JO - Brain Research Bulletin
JF - Brain Research Bulletin
SN - 0361-9230
IS - 6
ER -