Enhanced neuropeptide Y release in the hippocampus is associated with chronic seizure susceptibility in kainic acid treated rats

Annamaria Vezzani, Gianluca Civenni, Massimo Rizzi, Antonella Monno, Silvia Messali, Rosario Samanin

Research output: Contribution to journalArticle

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Abstract

We measured the release of neuropeptide Y (NPY) from hippocampal slices of rats at various times after limbic seizures induced by a subcutaneous injection of 12 mg/kg kainic acid (KA). Two days after KA, 100 mM KCl induced a 1.6±0.2-fold increase in NPY release compared to saline-injected rats (P <0.05), while spontaneous and 50 mM KCl-induced release were unchanged. Thirty days after KA, the spontaneous and 100 mM KCl-induced efflux of NPY was enhanced 2-fold on average (P <0.01) compared to controls, while no significant differences were found using 50 mM KCl. Tissue concentration of NPY was raised 2.2 ± 0.02 times (P <0.01) 30 days after KA. Thirty days after KA, the rats showed enhanced susceptibility to tonic-clonic seizures, assessed using a normally subconvulsive dose of pentylenetetrazol (PTZ; 30 mg/kg). A selective antibody (Ab) raised against NPY in a rabbit was infused bilaterally for three days in the CA3 area and dentate gyrus (DG) of the dorsal hippocampus of rats treated 30 days before with KA. This significantly reduced (P <0.05) the number of animals with tonic-clonic seizures induced by 30 mg/kg PTZ, compared to KA treated rats which received the inactivated Ab. The Ab was ineffective in naive rats injected with a full convulsive dose of PTZ (55 mg/kg). The present results show that neuronal release of NPY is enhanced in the hippocampus after limbic induced in rats by KA. This effect persists for at least 30 days and may contribute to the chronically enhanced susceptibility to seizures after injection of this toxin.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalBrain Research
Volume660
Issue number1
DOIs
Publication statusPublished - Oct 10 1994

Fingerprint

Kainic Acid
Neuropeptide Y
Hippocampus
Seizures
Antibodies
Parahippocampal Gyrus
Pentylenetetrazole
Dentate Gyrus
Subcutaneous Injections
Rabbits
Injections

Keywords

  • Hippocampus
  • In vitro release
  • Neuropeptide Y
  • Rat
  • Seizure
  • Sprouting
  • Temporal lobe epilepsy

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Enhanced neuropeptide Y release in the hippocampus is associated with chronic seizure susceptibility in kainic acid treated rats. / Vezzani, Annamaria; Civenni, Gianluca; Rizzi, Massimo; Monno, Antonella; Messali, Silvia; Samanin, Rosario.

In: Brain Research, Vol. 660, No. 1, 10.10.1994, p. 138-143.

Research output: Contribution to journalArticle

Vezzani, Annamaria ; Civenni, Gianluca ; Rizzi, Massimo ; Monno, Antonella ; Messali, Silvia ; Samanin, Rosario. / Enhanced neuropeptide Y release in the hippocampus is associated with chronic seizure susceptibility in kainic acid treated rats. In: Brain Research. 1994 ; Vol. 660, No. 1. pp. 138-143.
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