Enhanced thromboxane biosynthesis in patients with chronic obstructive pulmonary disease

Giovanni Davì, Stefania Basili, Marco Vieri, Francesco Cipollone, Stella Santarone, Cesare Alessandri, Pierpaolo Gazzaniga, Corrado Cordova, Francesco Violi, N. De Luca, L. Coppotelli, M. Paradiso, A. Bellomo, A. Belogi, P. Ferroni, F. Pulcinelli, S. Roccaforte, T. Antidormi

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Abstract

Thrombotic complications of pulmonary circulation occur in patients with chronic obstructive pulmonary disease (COPD). In the present study, we sought to evaluate in vivo platelet activation through the measurement of 11- dehydro-thromboxane (Tx) B2, TxA2 major metabolite in the urine, in 29 patients with COPD, compared with 29 sex- and age-matched healthy subjects. The urinary excretion of 11-dehydro-TxB2 was significantly higher in patients with COPD than in control subjects: median (range), 753 (277-4,409) and 275 (129-612) pg/mg creatinine, respectively; p <0.0001). Moreover, 11- dehydro-TxB2 excretion was inversely related with arterial oxygen tension (rho = -0.46; p = 0.0145). In five of the 29 patients a short-term therapeutic course with oxygen supplementation induced a significant decrease of urinary 11-dehydro-TxB2 excretion: median range, 941 (452-2,640) to 445 (166-1,560) pg/mg creatinine. Moreover, selective inhibition of platelet cyclooxygenase activity by low-dose aspirin was associated with more than 90% inhibition of thromboxane metabolite excretion, demonstrating its being of platelet origin. Plasma levels of prothrombin fragment F1+2 were higher in patients than in control subjects (2.6 ± 1.5 versus 0.9 ± 0.4 nM, p = 0.0001). No relation between 11-dehydro-TxB2 excretion and plasma F1+2 levels was found. We conclude that platelet TxA2 biosynthesis is enhanced in patients with COPD and may be influenced by arterial oxygen tension changes.

Original languageEnglish
Pages (from-to)1794-1799
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume156
Issue number6
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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    Davì, G., Basili, S., Vieri, M., Cipollone, F., Santarone, S., Alessandri, C., Gazzaniga, P., Cordova, C., Violi, F., De Luca, N., Coppotelli, L., Paradiso, M., Bellomo, A., Belogi, A., Ferroni, P., Pulcinelli, F., Roccaforte, S., & Antidormi, T. (1997). Enhanced thromboxane biosynthesis in patients with chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine, 156(6), 1794-1799.