Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells

M. Gramse, F. Breviario, G. Pintucci, I. Millet, E. Dejana, J. van Damme, M. B. Donati, L. Mussoni

Research output: Contribution to journalArticlepeer-review

Abstract

Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alfa (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an apparent m.w. of 53 Kd and inhibited both tissue-type plasminogen activator (t-PA) and urokinase (UK). No change in t-PA antigen level was detected with any of the IL-1 preparations tested.

Original languageEnglish
Pages (from-to)720-727
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number2
DOIs
Publication statusPublished - Sep 16 1986

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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