Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells

M. Gramse; F. Breviario; G. Pintucci; I. Millet; E. Dejana; J. van Damme; M. B. Donati; L. Mussoni

doi:10.1016/S0006-291X(86)80050-X

Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells

M. Gramse, F. Breviario, G. Pintucci, I. Millet, E. Dejana, J. van Damme, M. B. Donati, L. Mussoni

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Abstract

Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alfa (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an apparent m.w. of 53 Kd and inhibited both tissue-type plasminogen activator (t-PA) and urokinase (UK). No change in t-PA antigen level was detected with any of the IL-1 preparations tested.

Original language	English
Pages (from-to)	720-727
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	139
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80050-X
Publication status	Published - Sep 16 1986

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0006-291X(86)80050-X

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title = "Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells",

abstract = "Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alfa (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an apparent m.w. of 53 Kd and inhibited both tissue-type plasminogen activator (t-PA) and urokinase (UK). No change in t-PA antigen level was detected with any of the IL-1 preparations tested.",

author = "M. Gramse and F. Breviario and G. Pintucci and I. Millet and E. Dejana and {van Damme}, J. and Donati, {M. B.} and L. Mussoni",

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T1 - Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells

AU - Gramse, M.

AU - Breviario, F.

AU - Pintucci, G.

AU - Millet, I.

AU - Dejana, E.

AU - van Damme, J.

AU - Donati, M. B.

AU - Mussoni, L.

PY - 1986/9/16

Y1 - 1986/9/16

N2 - Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alfa (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an apparent m.w. of 53 Kd and inhibited both tissue-type plasminogen activator (t-PA) and urokinase (UK). No change in t-PA antigen level was detected with any of the IL-1 preparations tested.

AB - Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alfa (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an apparent m.w. of 53 Kd and inhibited both tissue-type plasminogen activator (t-PA) and urokinase (UK). No change in t-PA antigen level was detected with any of the IL-1 preparations tested.

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Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells

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