Enhancement of a culture of human osteoblasts inside hydroxyapatite scaffolds via [2 mT; 75 Hz]-electromagnetic bioreactor

L. Fassina, E. Saino, L. Visai, M. G Cusella De Angelis, F. Benazzo, G. Magenes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is commonly employed owing to its biocompatibility; in addition , the in vitro modification of hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering . In this study we have followed a biomimetic strategy where electromagnetically stimulated SAOS -2 human osteoblasts proliferated and built their extracellular matrix inside porous hydroxyapatite. In comparison with control conditions , the electromagnetic stimulus (magnetic field , 2 mT; frequency , 75 Hz) increased , in vitro, the cell proliferation and the coating of hydroxyapatite with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The physical stimulus aimed at obtaining a better in vitro modification of porous hydroxyapatite in terms of cell colonization and coating with osteogenic signals , like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages87-90
Number of pages4
Volume25
Edition10
DOIs
Publication statusPublished - 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs - Munich, Germany
Duration: Sep 7 2009Sep 12 2009

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs
CountryGermany
CityMunich
Period9/7/099/12/09

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Keywords

  • Biomimetics
  • Bone extracellular matrix
  • Cell proliferation
  • Electromagnetic stimulation
  • Hydroxyapatite
  • Osteoblast
  • Surface modification

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

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