Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs

Angelo Martino, Rita Casetti, Fabrizio Poccia

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.

Original languageEnglish
Pages (from-to)1023-1029
Number of pages7
JournalVaccine
Volume25
Issue number6
DOIs
Publication statusPublished - Jan 22 2007

Fingerprint

Mycobacterium bovis
dendritic cells
Dendritic Cells
T-lymphocytes
immune response
zoledronic acid
T-Lymphocytes
drugs
Pharmaceutical Preparations
Immunity
immunity
Interleukin-15
interleukin-12
coculture
Interleukin-12
interleukin-10
Coculture Techniques
Interleukin-10

Keywords

  • γδ
  • BCG
  • Dendritic cells

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs. / Martino, Angelo; Casetti, Rita; Poccia, Fabrizio.

In: Vaccine, Vol. 25, No. 6, 22.01.2007, p. 1023-1029.

Research output: Contribution to journalArticle

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