Abstract
Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.
| Original language | English |
|---|---|
| Pages (from-to) | 1023-1029 |
| Number of pages | 7 |
| Journal | Vaccine |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jan 22 2007 |
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Keywords
- γδ
- BCG
- Dendritic cells
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs. / Martino, Angelo; Casetti, Rita; Poccia, Fabrizio.
In: Vaccine, Vol. 25, No. 6, 22.01.2007, p. 1023-1029.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs
AU - Martino, Angelo
AU - Casetti, Rita
AU - Poccia, Fabrizio
PY - 2007/1/22
Y1 - 2007/1/22
N2 - Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.
AB - Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.
KW - γδ
KW - BCG
KW - Dendritic cells
UR - http://www.scopus.com/inward/record.url?scp=33845568804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845568804&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2006.09.070
DO - 10.1016/j.vaccine.2006.09.070
M3 - Article
C2 - 17118497
AN - SCOPUS:33845568804
VL - 25
SP - 1023
EP - 1029
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 6
ER -