Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs

Angelo Martino; Rita Casetti; Fabrizio Poccia

doi:10.1016/j.vaccine.2006.09.070

Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs

Angelo Martino, Rita Casetti, Fabrizio Poccia

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article

Abstract

Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.

Original language	English
Pages (from-to)	1023-1029
Number of pages	7
Journal	Vaccine
Volume	25
Issue number	6
DOIs	https://doi.org/10.1016/j.vaccine.2006.09.070
Publication status	Published - Jan 22 2007

Keywords

γδ
BCG
Dendritic cells

ASJC Scopus subject areas

Immunology
Microbiology
Virology
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)

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Martino, A., Casetti, R., & Poccia, F. (2007). Enhancement of BCG-induced Th1 immune response through Vγ9Vδ2 T cell activation with non-peptidic drugs. Vaccine, 25(6), 1023-1029. https://doi.org/10.1016/j.vaccine.2006.09.070

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abstract = "Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on na{\"i}ve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.",

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N2 - Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.

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