Abstract
Since drug-activated γδ T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining γδ T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated γδ T cells. Specifically, the co-culture with activated Vγ9Vδ2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-α and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by γδ T cell activation with BrHpp or Zol. These data indicate that γδ T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.
Original language | English |
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Pages (from-to) | 1023-1029 |
Number of pages | 7 |
Journal | Vaccine |
Volume | 25 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jan 22 2007 |
Keywords
- γδ
- BCG
- Dendritic cells
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)