Enhancement of fibroblast proliferation, collagen biosynthesis and production of growth factors as a result of combining sodium hyaluronate and aminoacids

Maria A. Mariggiò, A. Cassano, A. Vinella, A. Vincenti, R. Fumarulo, L. Lo Muzio, E. Maiorano, D. Ribatti, G. Favia

Research output: Contribution to journalArticle

Abstract

Fibroblasts play a key role in tissue healing by producing the majority of extracellular matrix components, favouring granulation tissue formation, and stimulating re-epithelialization. Hyaluronan is a component of ECM and its anti-inflammatory effects and properties in enhancing wound closure are well known. In this study, we examined the effects of Aminogam® gel, a new pharmacological preparation suggested to improve wound healing, composed of hyaluronic acid, proline, lysine, glycine and leucine, on human fibroblasts. Results show that fibroblasts treated with hyaluronic acid plus aminoacid solution increased their proliferative activity, collagen I and III, and fibronectin synthesis. Moreover, HA plus aminoacid solution increased the expression of transforming growth factor beta, connective tissue growth factor, interleukin-6 and -8, assayed by RT-PCR. These results suggested that Aminogam® gel, involved in several stages of wound healing, as fibroblast proliferation, granulation tissue formation, ECM component deposition, and production of cytokines, may be a useful device to favour and accelerate wound closure.

Original languageEnglish
Pages (from-to)485-492
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume22
Issue number2
Publication statusPublished - Apr 2009

    Fingerprint

Keywords

  • Aminoacids
  • Fibroblasts
  • Hyaluronan
  • Wound healing

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

Mariggiò, M. A., Cassano, A., Vinella, A., Vincenti, A., Fumarulo, R., Lo Muzio, L., Maiorano, E., Ribatti, D., & Favia, G. (2009). Enhancement of fibroblast proliferation, collagen biosynthesis and production of growth factors as a result of combining sodium hyaluronate and aminoacids. International Journal of Immunopathology and Pharmacology, 22(2), 485-492.