Enhancement of hyperthermic damage on M14-melanoma cells by liposome pretreatment

N. Laudonio, L. Marcocci, G. Arancia, A. Calcabrini, D. Del Bufalo, C. Greco, G. Zupi, I. Mavelli, J. Z. Pedersen, A. Bozzi, B. Mondovi

Research output: Contribution to journalArticlepeer-review


Exponentially growing human melanoma cells (M14 cell line) were pretreared with various amounts of dipalmitoylphosphatidylcholine-containing multilamellar liposomes and then exposed to heat treatment (42.5°C). Cell damage produced by the treatments, given separately or in combination, was evaluated in terms of cell survival. Our results demonstrate that the cell survival at 37°C was not affected by liposome concentrations up to 1000 nmol of phospholipid/2.5 × 106 cells, while liposome treatment of cells before heat exposure determined a marked damaging effect even at 100 nmol of phospholipid/2.5 × 106 cells. The mechanisms of liposome-cell interaction have been investigated by electron microscopy or by electron spin resonance measurements of spin-labeled membranes of intact cells. Evidence has been obtained that liposomal lipids are either taken up by M14 cells or become incorporated in the cell membrane. The present data suggest the possibility that liposome treatments per se could be of potential value as a therapeutic approach, by increasing the effect of heat therapy.

Original languageEnglish
Pages (from-to)5119-5126
Number of pages8
JournalCancer Research
Issue number16
Publication statusPublished - Aug 15 1990

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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