Enhancement of lysine acetylation accelerates wound repair

Francesco Spallotta, Chiara Cencioni, Stefania Straino, Gianluca Sbardella, Sabrina Castellano, Maurizio C. Capogrossi, Fabio Martelli, Carlo Gaetano

Research output: Contribution to journalArticlepeer-review

Abstract

In physiopathological conditions, such as diabetes, wound healing is significantly compromised and chronic complications, including ulcers, may occur. In a mouse model of skin repair, we recently reported that wound treatment with Sirtuin activators and class I HDAC inhibitors induced keratinocyte proliferation and enhanced healing via a nitric oxide (NO) dependent mechanism. We observed an increase in total protein acetylation in the wound area, as determined by acetylation of a-tubulin and histone H3 Lysine 9. We reasoned that this process activated cell function as well as regulated gene expression to foster tissue repair. We report here that the direct activation of P300/CBP-associated factor (PCAF) by the histone acetylase activator pentadecylidenemalonate 1b (SPV-106) induced Lysine acetylation in the wound area. This intervention was sufficient to enhance repair process by a NO-independent mechanism. Hence, an impairment of PCAF and/or other GCN5 family acetylases may delay skin repair in physiopathological conditions.

Original languageEnglish
Article numbere25466
JournalCommunicative and Integrative Biology
Volume6
Issue number5
DOIs
Publication statusPublished - 2013

Keywords

  • Epigenetics
  • Keratinocyteskin
  • Lysine acetylation
  • Nitric oxide
  • PCAF
  • Wound healing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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