Abstract
| Original language | English |
|---|---|
| Article number | 1802886 |
| Journal | Small |
| Volume | 14 |
| Issue number | 45 |
| DOIs | |
| Publication status | Published - 2018 |
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Enhancement of Tumor Homing by Chemotherapy-Loaded Nanoparticles. / Ponzoni, M; Curnis, F; Brignole, C; Bruno, S; Guarnieri, Daniela; Sitia, L; Marotta, Roberto; Sacchi, A; Bauckneht, M; Buschiazzo, A; Rossi, A; Di Paolo, D; Perri, P; Gori, A; Sementa, AR; Emionite, L; Cilli, M; Tamma, R; Ribatti, D; Pompa, PP; Marini, C; Sambuceti, G; Corti, A; Pastorino, F.
In: Small, Vol. 14, No. 45, 1802886, 2018.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Enhancement of Tumor Homing by Chemotherapy-Loaded Nanoparticles
AU - Ponzoni, M
AU - Curnis, F
AU - Brignole, C
AU - Bruno, S
AU - Guarnieri, Daniela
AU - Sitia, L
AU - Marotta, Roberto
AU - Sacchi, A
AU - Bauckneht, M
AU - Buschiazzo, A
AU - Rossi, A
AU - Di Paolo, D
AU - Perri, P
AU - Gori, A
AU - Sementa, AR
AU - Emionite, L
AU - Cilli, M
AU - Tamma, R
AU - Ribatti, D
AU - Pompa, PP
AU - Marini, C
AU - Sambuceti, G
AU - Corti, A
AU - Pastorino, F
PY - 2018
Y1 - 2018
N2 - Targeted delivery of anticancer drugs with nanocarriers can reduce side effects and ameliorate therapeutic efficacy. However, poorly perfused and dysfunctional tumor vessels limit the transport of the payload into solid tumors. The use of tumor-penetrating nanocarriers might enhance tumor uptake and antitumor effects. A peptide containing a tissue-penetrating (TP) consensus motif, capable of recognizing neuropilin-1, is here fused to a neuroblastoma-targeting peptide (pep) previously developed. Neuroblastoma cell lines and cells derived from both xenografts and high-risk neuroblastoma patients show overexpression of neuropilin-1. In vitro studies reveal that TP–pep binds cell lines and cells derived from neuroblastoma patients more efficiently than pep. TP–pep, after coupling to doxorubicin-containing stealth liposomes (TP–pep–SL[doxorubicin]), enhances their uptake by cells and cytotoxic effects in vitro, while increasing tumor-binding capability and homing in vivo. TP–pep–SL[doxorubicin] treatment enhances the Evans Blue dye accumulation in tumors but not in nontumor tissues, pointing to selective increase of vascular permeability in tumor tissues. Compared to pep–SL[doxorubicin], TP–pep–SL[doxorubicin] shows an increased antineuroblastoma activity in three neuroblastoma animal models mimicking the growth of neuroblastoma in humans. The enhancement of drug penetration in tumors by TP–pep-targeted nanoparticles may represent an innovative strategy for neuroblastoma. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
AB - Targeted delivery of anticancer drugs with nanocarriers can reduce side effects and ameliorate therapeutic efficacy. However, poorly perfused and dysfunctional tumor vessels limit the transport of the payload into solid tumors. The use of tumor-penetrating nanocarriers might enhance tumor uptake and antitumor effects. A peptide containing a tissue-penetrating (TP) consensus motif, capable of recognizing neuropilin-1, is here fused to a neuroblastoma-targeting peptide (pep) previously developed. Neuroblastoma cell lines and cells derived from both xenografts and high-risk neuroblastoma patients show overexpression of neuropilin-1. In vitro studies reveal that TP–pep binds cell lines and cells derived from neuroblastoma patients more efficiently than pep. TP–pep, after coupling to doxorubicin-containing stealth liposomes (TP–pep–SL[doxorubicin]), enhances their uptake by cells and cytotoxic effects in vitro, while increasing tumor-binding capability and homing in vivo. TP–pep–SL[doxorubicin] treatment enhances the Evans Blue dye accumulation in tumors but not in nontumor tissues, pointing to selective increase of vascular permeability in tumor tissues. Compared to pep–SL[doxorubicin], TP–pep–SL[doxorubicin] shows an increased antineuroblastoma activity in three neuroblastoma animal models mimicking the growth of neuroblastoma in humans. The enhancement of drug penetration in tumors by TP–pep-targeted nanoparticles may represent an innovative strategy for neuroblastoma. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
U2 - 10.1002/smll.201802886
DO - 10.1002/smll.201802886
M3 - Article
VL - 14
JO - Small
JF - Small
SN - 1613-6810
IS - 45
M1 - 1802886
ER -