Abstract
Tumor-promoting agents are known to inhibit the specific differentiation processes of ceveral animal cell systems in vitro, including the Friend leukemia cell system. We have examined the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the latter system and have investigated its action on Friend virus expression. At a concentration of 16.7 nM, TPA inhibits the dimethyl sulfoxide-induced Friend cell terminal differentiation and, at the same time, enhances the expression of the Friend virus genome, as demonstrated by a 2-fold increase in the amount of reverse transcriptase-containing particles released into the culture fluid and in the levels of virus-specific intracytoplasmic RNA. The greatest effect of TPA is evident after 24 hr of treatment. At this time, TPA exerts also its strongest effect upon the induction of the plasminogen activator. Our results indicate that two specific effects of TPA, i.e., block of differentiation and induction of plasminogen activator, correlate well in the Friend cell system with an extracellular and intracellular increase in virus expression.
| Original language | English |
|---|---|
| Pages (from-to) | 3369-3373 |
| Number of pages | 5 |
| Journal | Cancer Research |
| Volume | 40 |
| Issue number | 9 |
| Publication status | Published - 1980 |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate. / Colletta, G.; Di Fiore, P. P.; Ferrentino, M.; Pietropaolo, C.; Turco, M. C.; Vecchio, G.
In: Cancer Research, Vol. 40, No. 9, 1980, p. 3369-3373.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate
AU - Colletta, G.
AU - Di Fiore, P. P.
AU - Ferrentino, M.
AU - Pietropaolo, C.
AU - Turco, M. C.
AU - Vecchio, G.
PY - 1980
Y1 - 1980
N2 - Tumor-promoting agents are known to inhibit the specific differentiation processes of ceveral animal cell systems in vitro, including the Friend leukemia cell system. We have examined the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the latter system and have investigated its action on Friend virus expression. At a concentration of 16.7 nM, TPA inhibits the dimethyl sulfoxide-induced Friend cell terminal differentiation and, at the same time, enhances the expression of the Friend virus genome, as demonstrated by a 2-fold increase in the amount of reverse transcriptase-containing particles released into the culture fluid and in the levels of virus-specific intracytoplasmic RNA. The greatest effect of TPA is evident after 24 hr of treatment. At this time, TPA exerts also its strongest effect upon the induction of the plasminogen activator. Our results indicate that two specific effects of TPA, i.e., block of differentiation and induction of plasminogen activator, correlate well in the Friend cell system with an extracellular and intracellular increase in virus expression.
AB - Tumor-promoting agents are known to inhibit the specific differentiation processes of ceveral animal cell systems in vitro, including the Friend leukemia cell system. We have examined the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the latter system and have investigated its action on Friend virus expression. At a concentration of 16.7 nM, TPA inhibits the dimethyl sulfoxide-induced Friend cell terminal differentiation and, at the same time, enhances the expression of the Friend virus genome, as demonstrated by a 2-fold increase in the amount of reverse transcriptase-containing particles released into the culture fluid and in the levels of virus-specific intracytoplasmic RNA. The greatest effect of TPA is evident after 24 hr of treatment. At this time, TPA exerts also its strongest effect upon the induction of the plasminogen activator. Our results indicate that two specific effects of TPA, i.e., block of differentiation and induction of plasminogen activator, correlate well in the Friend cell system with an extracellular and intracellular increase in virus expression.
UR - http://www.scopus.com/inward/record.url?scp=0018954586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018954586&partnerID=8YFLogxK
M3 - Article
C2 - 6159074
AN - SCOPUS:0018954586
VL - 40
SP - 3369
EP - 3373
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 9
ER -