Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate

G. Colletta, P. P. Di Fiore, M. Ferrentino, C. Pietropaolo, M. C. Turco, G. Vecchio

Research output: Contribution to journalArticle

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Abstract

Tumor-promoting agents are known to inhibit the specific differentiation processes of ceveral animal cell systems in vitro, including the Friend leukemia cell system. We have examined the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the latter system and have investigated its action on Friend virus expression. At a concentration of 16.7 nM, TPA inhibits the dimethyl sulfoxide-induced Friend cell terminal differentiation and, at the same time, enhances the expression of the Friend virus genome, as demonstrated by a 2-fold increase in the amount of reverse transcriptase-containing particles released into the culture fluid and in the levels of virus-specific intracytoplasmic RNA. The greatest effect of TPA is evident after 24 hr of treatment. At this time, TPA exerts also its strongest effect upon the induction of the plasminogen activator. Our results indicate that two specific effects of TPA, i.e., block of differentiation and induction of plasminogen activator, correlate well in the Friend cell system with an extracellular and intracellular increase in virus expression.

Original languageEnglish
Pages (from-to)3369-3373
Number of pages5
JournalCancer Research
Volume40
Issue number9
Publication statusPublished - 1980

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Leukemia, Erythroblastic, Acute
Viral Genes
Tetradecanoylphorbol Acetate
Gene Expression
Friend murine leukemia virus
Plasminogen Activators
Viruses
RNA-Directed DNA Polymerase
Dimethyl Sulfoxide
Cell Differentiation
Leukemia
Genome
RNA
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Colletta, G., Di Fiore, P. P., Ferrentino, M., Pietropaolo, C., Turco, M. C., & Vecchio, G. (1980). Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate. Cancer Research, 40(9), 3369-3373.

Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate. / Colletta, G.; Di Fiore, P. P.; Ferrentino, M.; Pietropaolo, C.; Turco, M. C.; Vecchio, G.

In: Cancer Research, Vol. 40, No. 9, 1980, p. 3369-3373.

Research output: Contribution to journalArticle

Colletta, G, Di Fiore, PP, Ferrentino, M, Pietropaolo, C, Turco, MC & Vecchio, G 1980, 'Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate', Cancer Research, vol. 40, no. 9, pp. 3369-3373.
Colletta, G. ; Di Fiore, P. P. ; Ferrentino, M. ; Pietropaolo, C. ; Turco, M. C. ; Vecchio, G. / Enhancement of viral gene expression in Friend erythroleukemia cells by 12-O-tetradecanoylphorbol-13-acetate. In: Cancer Research. 1980 ; Vol. 40, No. 9. pp. 3369-3373.
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