Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route

Emanuele Cocucci, Gabriella Racchetti, Paola Podini, Marjan Rupnik, Jacopo Meldolesi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Enlargeosomes, a new type of widely expressed cytoplasmic vesicles, undergo tetanus toxin-insensitive exocytosis in response to cytosolic Ca2+ concentration ([Ca2+]i) rises. Cell biology of enlargeosomes is still largely unknown. By combining immunocytochemistry (marker desmoyokin-Ahnak, d/A) to capacitance electrophysiology in the enlargeosome-rich, neurosecretion-defective clone PC12-27, we show that 1) the two responses, cell surface enlargement and d/A surface appearance, occur with similar kinetics and in the same low micromolar [Ca2+]i range, no matter whether induced by photolysis of the caged Ca2+ compound o-nitrophenyl EGTA or by the Ca2+ ionophore ionomycin. Thus, enlargeosomes seem to account, at least in large part, for the exocytic processes triggered by the two stimulations. 2. The enlargeosome membranes are resistant to nonionic detergents but distinct from other resistant membranes, rich in caveolin, Thy1, and/or flotillin1. 3. Cell cholesterol depletion, which affects many membrane fusions, neither disrupts enlargeosomes nor affects their regulated exocytosis. 4. The postexocytic cell surface decline is [Ca 2+]i dependent. 5. Exocytized d/A-rich membranes are endocytized and trafficked along an intracellular pathway by nonacidic organelles, distinct from classical endosomes and lysosomes. Our data define specific aspects of enlargeosomes and suggest their participation, in addition to cell differentiation and repair, for which evidence already exists, to other physiological and pathological processes.

Original languageEnglish
Pages (from-to)5356-5368
Number of pages13
JournalMolecular Biology of the Cell
Volume15
Issue number12
DOIs
Publication statusPublished - Dec 2004

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Endocytosis
Detergents
Exocytosis
Membranes
Cell Enlargement
Neurosecretion
Cytoplasmic Vesicles
Physiological Phenomena
Caveolins
Tetanus Toxin
Ionomycin
Membrane Fusion
Photolysis
Electrophysiology
Endosomes
Ionophores
Pathologic Processes
Lysosomes
Organelles
Cell Biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route. / Cocucci, Emanuele; Racchetti, Gabriella; Podini, Paola; Rupnik, Marjan; Meldolesi, Jacopo.

In: Molecular Biology of the Cell, Vol. 15, No. 12, 12.2004, p. 5356-5368.

Research output: Contribution to journalArticle

Cocucci, Emanuele ; Racchetti, Gabriella ; Podini, Paola ; Rupnik, Marjan ; Meldolesi, Jacopo. / Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route. In: Molecular Biology of the Cell. 2004 ; Vol. 15, No. 12. pp. 5356-5368.
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