Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells

E. Resca, M. Zavatti, T. Maraldi, L. Bertoni, F. Beretti, M. Guida, G. B. La Sala, P. V. Guillot, A. L. David, N. J. Sebire, A. De Pol, P. De Coppi

Research output: Contribution to journalArticle

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Abstract

Introduction Human term placenta has attracted increasing attention as an alternative source of stem cells for regenerative medicine since it is accessible without ethical objections. The amniotic membrane (AM) contains at least two stem cell types from different embryological origins: ectodermal amniotic epithelial stem cells, and mesodermal mesenchymal stromal cells. Among the second group we studied the characteristics of amniotic mesenchymal cells (AMC) versus the ones enriched for the commonly used surface marker c-Kit (amniotic progenitor/stem cells-ASC), a stem cell factor receptor with crucial functions in a variety of biological systems and presents in early progenitors of different origin, as been already demonstrated in the enriched chorionic stem cells.

Methods After isolation, cells from the amniotic membranes (amniotic cells-AC) were selected for c-Kit (ASC) and compared these cells with c-Kit unselected (AMC), evaluating the expression of other stem cell markers (Oct-4, Tra-1-81, SSEA-4), CD271 and Slug.

Results Immunofluorescence analysis showed that ASC cells exhibited greater stem cell marker expression and included more CD271 and Slug positive cells. This was consistent with the interpretation that c-Kit enriched AC show greater stemness capacity compared to c-Kit unselected AMC.

Discussion AMC and ASC can both differentiate into various cell types including adipogenic, osteogenic, chondrogenic, neurogenic and hepatic lineages, but the enrichment in c-Kit improved stemness and differentiation potential of ASC.

Original languageEnglish
Pages (from-to)18-26
Number of pages9
JournalPlacenta
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Amnion
Stem Cells
Gastropoda
Proto-Oncogene Proteins c-kit
Regenerative Medicine
Mesenchymal Stromal Cells
Placenta
Fluorescent Antibody Technique
Epithelial Cells
Cell Membrane
Liver

Keywords

  • Amniotic membrane
  • c-Kit
  • Human amniotic stem cells
  • Oct-4
  • Placenta
  • Pluripotency markers

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Resca, E., Zavatti, M., Maraldi, T., Bertoni, L., Beretti, F., Guida, M., ... De Coppi, P. (2015). Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells. Placenta, 36(1), 18-26. https://doi.org/10.1016/j.placenta.2014.11.002

Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells. / Resca, E.; Zavatti, M.; Maraldi, T.; Bertoni, L.; Beretti, F.; Guida, M.; La Sala, G. B.; Guillot, P. V.; David, A. L.; Sebire, N. J.; De Pol, A.; De Coppi, P.

In: Placenta, Vol. 36, No. 1, 01.01.2015, p. 18-26.

Research output: Contribution to journalArticle

Resca, E, Zavatti, M, Maraldi, T, Bertoni, L, Beretti, F, Guida, M, La Sala, GB, Guillot, PV, David, AL, Sebire, NJ, De Pol, A & De Coppi, P 2015, 'Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells', Placenta, vol. 36, no. 1, pp. 18-26. https://doi.org/10.1016/j.placenta.2014.11.002
Resca, E. ; Zavatti, M. ; Maraldi, T. ; Bertoni, L. ; Beretti, F. ; Guida, M. ; La Sala, G. B. ; Guillot, P. V. ; David, A. L. ; Sebire, N. J. ; De Pol, A. ; De Coppi, P. / Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells. In: Placenta. 2015 ; Vol. 36, No. 1. pp. 18-26.
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AU - Beretti, F.

AU - Guida, M.

AU - La Sala, G. B.

AU - Guillot, P. V.

AU - David, A. L.

AU - Sebire, N. J.

AU - De Pol, A.

AU - De Coppi, P.

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