TY - JOUR
T1 - Enrichment of histones from patient samples for mass spectrometry-based analysis of post-translational modifications
AU - Noberini, Roberta
AU - Restellini, Camilla
AU - Savoia, Evelyn Oliva
AU - Bonaldi, Tiziana
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Aberrations in histone post-translational modifications (PTMs) have been implicated with the development of numerous pathologies, including cancer. Therefore, profiling histone PTMs in patient samples could provide information useful for the identification of epigenetic biomarkers, as well as for the discovery of potential novel targets. While antibody-based methods have been traditionally employed to analyze histone PTM in clinical samples, mass spectrometry (MS) can provide a more comprehensive, unbiased and quantitative view on histones and their PTMs. To combine the power of MS-based methods and the potential offered by histone PTM profiling of clinical samples, we have recently developed a series of methods for the extraction and enrichment of histones from different types of patient samples, including formalin-fixed paraffin-embedded tissues, fresh- and optimal cutting temperature-frozen tissues, and primary cells. Here, we provide a detailed description of these protocols, together with indications on the expected results and the most suitable workflow to be used downstream of each procedure.
AB - Aberrations in histone post-translational modifications (PTMs) have been implicated with the development of numerous pathologies, including cancer. Therefore, profiling histone PTMs in patient samples could provide information useful for the identification of epigenetic biomarkers, as well as for the discovery of potential novel targets. While antibody-based methods have been traditionally employed to analyze histone PTM in clinical samples, mass spectrometry (MS) can provide a more comprehensive, unbiased and quantitative view on histones and their PTMs. To combine the power of MS-based methods and the potential offered by histone PTM profiling of clinical samples, we have recently developed a series of methods for the extraction and enrichment of histones from different types of patient samples, including formalin-fixed paraffin-embedded tissues, fresh- and optimal cutting temperature-frozen tissues, and primary cells. Here, we provide a detailed description of these protocols, together with indications on the expected results and the most suitable workflow to be used downstream of each procedure.
KW - Epigenetics
KW - Formalin-fixed paraffin-embedded tissues
KW - Frozen tissues
KW - Histone post-translational modifications
KW - Mass spectrometry
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U2 - 10.1016/j.ymeth.2019.10.001
DO - 10.1016/j.ymeth.2019.10.001
M3 - Article
C2 - 31605746
AN - SCOPUS:85075346683
JO - ImmunoMethods
JF - ImmunoMethods
SN - 1046-2023
ER -