Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 19-28 |
| Number of pages | 10 |
| Journal | Methods |
| Volume | 184 |
| DOIs | |
| Publication status | Published - 2020 |
Keywords
- Epigenetics
- Formalin-fixed paraffin-embedded tissues
- Frozen tissues
- Histone post-translational modifications
- Mass spectrometry
- animal tissue
- Article
- controlled study
- histone modification
- human
- human cell
- human tissue
- immunohistochemistry
- laser microdissection
- male
- mass spectrometry
- nonhuman
- polyacrylamide gel electrophoresis
- practice guideline
- primary cell
- priority journal
- protein processing
- tissue embedding
- tissue section
- workflow
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Enrichment of histones from patient samples for mass spectrometry-based analysis of post-translational modifications. / Noberini, R.; Restellini, C.; Savoia, E.O.; Bonaldi, T.
In: Methods, Vol. 184, 2020, p. 19-28.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Enrichment of histones from patient samples for mass spectrometry-based analysis of post-translational modifications
AU - Noberini, R.
AU - Restellini, C.
AU - Savoia, E.O.
AU - Bonaldi, T.
N1 - Cited By :4 Export Date: 4 March 2021 CODEN: MTHDE Correspondence Address: Noberini, R.; Department of Experimental Oncology, Italy; email: roberta.noberini@ieo.it Funding details: GR-2016-02361522 Funding details: Ecumenical Project for International Cooperation, EPIC, 823839 Funding details: Horizon 2020 Framework Programme, H2020 Funding details: European Commission***Delivered and deleted from Elsevier end because this record is to be no longer updated or in business with Elsevier on Date 10-03-2020***, EC Funding details: Associazione Italiana per la Ricerca sul Cancro, AIRC, IG-2018-21834 Funding text 1: Funding: This research was supported by the Italian Association for Cancer Research , grant number IG-2018-21834 (to T.B.), by EPIC -XS, project number 823839 , funded by the Horizon 2020 programme of the European Union (to T.B.), and by the Italian Ministry of Health, grant number GR-2016-02361522 (to R.N.). Appendix A Funding text 2: Funding: This research was supported by the Italian Association for Cancer Research, grant number IG-2018-21834 (to T.B.), by EPIC-XS, project number 823839, funded by the Horizon 2020 programme of the European Union (to T.B.), and by the Italian Ministry of Health, grant number GR-2016-02361522 (to R.N.). References: Zhao, Y., Garcia, B.A., Comprehensive Catalog of Currently Documented Histone Modifications (2015) Cold Spring Harb. Perspect. Biol., 7 (9); Audia, J.E., Campbell, R.M., Histone Modifications and Cancer (2016) Cold Spring Harb. Perspect. Biol., 8 (4); Portela, A., Esteller, M., Epigenetic modifications and human disease (2010) Nat. 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PY - 2020
Y1 - 2020
N2 - Aberrations in histone post-translational modifications (PTMs) have been implicated with the development of numerous pathologies, including cancer. Therefore, profiling histone PTMs in patient samples could provide information useful for the identification of epigenetic biomarkers, as well as for the discovery of potential novel targets. While antibody-based methods have been traditionally employed to analyze histone PTM in clinical samples, mass spectrometry (MS) can provide a more comprehensive, unbiased and quantitative view on histones and their PTMs. To combine the power of MS-based methods and the potential offered by histone PTM profiling of clinical samples, we have recently developed a series of methods for the extraction and enrichment of histones from different types of patient samples, including formalin-fixed paraffin-embedded tissues, fresh- and optimal cutting temperature-frozen tissues, and primary cells. Here, we provide a detailed description of these protocols, together with indications on the expected results and the most suitable workflow to be used downstream of each procedure. © 2019 The Authors
AB - Aberrations in histone post-translational modifications (PTMs) have been implicated with the development of numerous pathologies, including cancer. Therefore, profiling histone PTMs in patient samples could provide information useful for the identification of epigenetic biomarkers, as well as for the discovery of potential novel targets. While antibody-based methods have been traditionally employed to analyze histone PTM in clinical samples, mass spectrometry (MS) can provide a more comprehensive, unbiased and quantitative view on histones and their PTMs. To combine the power of MS-based methods and the potential offered by histone PTM profiling of clinical samples, we have recently developed a series of methods for the extraction and enrichment of histones from different types of patient samples, including formalin-fixed paraffin-embedded tissues, fresh- and optimal cutting temperature-frozen tissues, and primary cells. Here, we provide a detailed description of these protocols, together with indications on the expected results and the most suitable workflow to be used downstream of each procedure. © 2019 The Authors
KW - Epigenetics
KW - Formalin-fixed paraffin-embedded tissues
KW - Frozen tissues
KW - Histone post-translational modifications
KW - Mass spectrometry
KW - animal tissue
KW - Article
KW - controlled study
KW - histone modification
KW - human
KW - human cell
KW - human tissue
KW - immunohistochemistry
KW - laser microdissection
KW - male
KW - mass spectrometry
KW - nonhuman
KW - polyacrylamide gel electrophoresis
KW - practice guideline
KW - primary cell
KW - priority journal
KW - protein processing
KW - tissue embedding
KW - tissue section
KW - workflow
U2 - 10.1016/j.ymeth.2019.10.001
DO - 10.1016/j.ymeth.2019.10.001
M3 - Article
VL - 184
SP - 19
EP - 28
JO - Methods
JF - Methods
SN - 1046-2023
ER -