Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge

N Clementi, E Criscuolo, F Cappelletti, P Quaranta, M Pistello, RA Diotti, GA Sautto, AW Tarr, F Mailland, D Concas, R Burioni, M Clementi, N Mancini

Research output: Contribution to journalArticlepeer-review

Abstract

The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed. Finally, mice receiving a single systemic injection of Hu-mAb#33 were protected from death and severe clinical manifestations following both ocular and vaginal HSV-1 and -2 lethal challenge. These results pave the way for further studies reassessing the importance of HSV entry as a novel target for therapeutic intervention and inhibition of cell-to-cell virus transmission. © 2017 Elsevier B.V.
Original languageEnglish
Pages (from-to)48-61
Number of pages14
JournalAntiviral Research
Volume143
Issue number5
DOIs
Publication statusPublished - 2017

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